同尾酶汇总表.docx

1、同尾酶汇总表连接互相匹配的粘性末端，产生新的酶切位点通常连接互相匹配的粘性末端可以产生新的酶切位点。以下组合是通过计算机程序计算岀来的，尽管我们希望能尽量保证其准确性， 但有些未经实验证实，因此不能保证100%的可行性。如果有同裂酶存在，则只列出其中的一个酶。本表中列出的内切酶均为已商品化 的内切酶。识别序列不够严谨的内切酶（例如，识别多个序列的酶）只在括号内标出相关的序列，但注意这些酶不只切割该序列。表示连接产物不能被再切割。酶连接到可进行切割的酶Acc65 1Ban 1 (G/GTACC)Acc65 I, Ban I, Kpn I, Nla IV, Rsa I(G/GTACC)BsiW I

2、, BsrG 1Rsa IAcc 1(GT/CGAC)Acil, Acl 1, BsaH 1 (GR/CGYC), HinP1 I, Hpa II, Nar I(GT/CGAC)Cla I, BstB I, Taq ITaq IAci 1Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I(C/CGC)BsaH I (GR/CGCC), HinP1 lf Nar IAci IHpa IIHpa IIAcl 1 (AA/CGTT)Acc I (GT/CGAC), Aci I, Cla I, BstB I, HinP1 I, Hpa II, Nar I, Taq

3、 IAfl III(A/CGCGT)Asc 1, BssH IIBstU I, Hha I(A/CATGT)BspH 1, Neo 1, Pci 1Nlalll(A/CGCGT)Mlu 1Afl III, BstU I, Mlu IAge 1 (A/CCGGT)Ava 1 (C/CCGGG), Xma 1Hpa II, Nci I, ScrF IBsaW 1, BspE 1BsaW I, Hpa IIBsrF 1 (A/CCGGT), SgrA 1 (CA/CCGGTG)Age I, BsaW I, BsrF I, Hpa IINgoM IVBsrF I, Hpa IIApa 1 (GGGCC

4、/C)Ban II (GGGCC/C), Bsp1286 I (GGGCC/C)Apa I, Ban II, Bsp120 I, Bsp1286 I, Hae III, Nla IV,Sau96 IApaL 1 (G/TGCAC)Sfc I (C/TGCAG)Bsg IApo 1(A/AATTY)EcoRIApo I, Tsp509 I(G/AATTY)EcoRIApo I, EcoR I, Tsp509 I(R/AATTY)Mfe I, Tsp509 ITsp509 IAsc 1(GG/CGCGCC)Afl III (A/CGCGT), Mlu IBstU I, Hha IBssH IIBs

5、sH II, BstU I, Cac8 I, Hha IAse 1 (AT/TAAT)Bfa I, Csp6 I, Nde IMse IMse IAsiS 1(GCGAT/CGC)BsiE I (CGAT/CG)Dpn II, Pvu IPadMse IPvu 1Dpn II, Pvu 1Ava 1(C/CCGGG)Age 1, BsaW 1, BspE 1, BsrF 1 (R/CCGGY), NgoM IV, SgrA 1 (CR/CCGGYG)Hpa II, Nci 1, ScrF 1(C/TCGAG)XholAva 1, Taq 1, Xho 1(C/TCGAG)SallTaq 1(C

6、/CCGGG)Xma 1Ava 1, BsaJ 1, Hpa II, Nci 1, ScrF 1, Sma 1Ava II(G/GWCC)PpuM 1 (RG/GACCY)Ava II, Nla IV, Sau96 1Rsr IIAva II, Sau96 1PpuM 1 (RG/GTCCY)Ava II, BsmF 1, Nla IV, Sau96 1Avril(C/CTAGG)Nhe L Spe 1, Xba 1BfalSty 1 (C/CTAGG)Avr II, Bfa 1, BsaJ 1, Sty 1BamH 1 (G/GATCC)Bel 1, Dpn IIAlw 1, Dpn IIB

7、gl II, BstY 1 (R/GATCY)Alw 1, BstY 1, Dpn IIBstY 1 (G/GATCC)Alw 1, BamH 1, BstY 1, Dpn II, Nla IVBan 1(G/GTACC)Acc65 1Acc65 1, Ban 1, Kpn 1, Nla IV, Rsa 1(G/GCGCC)KaslBan 1, BsaH 1, Hae II, Hha 1, Kas 1, Nar 1, Nla IV(G/GTACC)BsiW L BsrG 1Rsa 1Ban II(GGGCC/C)Apa 1, Bsp1286 I (GGGCC/C)Apa I, Ban II,

8、Bsp1286 I, Hae III, Nla IV, Sau96 I(GAGCT/C)Bsp1286 I (GAGCT/C), Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac IBel 1 仃 /GATCA)BamH I, BstY I (R/GATCY)Alw I, Dpn IIBgl II, Mbo IIDpn IIBfal (C/TAG)Ase I, Csp6 I, Mse I, Nde I-Bglll (A/GATCT)BamH I, BstY I (R/GATCY)Alw I, BstY I, Dpn IIBel I, Dpn IIDpn

9、IIBsaH 1(GR/CGYC)Acc I (GT/CGAC), Cla I, BstB I, Taq I(GA/CGYC)Acil, HinP1 IHgal(GG/CG YC)Aci I, HinP1 IHhal(GG/CG YC)Hpa IIAci I(GA/CGYC)Nar IBsaH I, Hga I(GG/CGYC)Nar IBan I, BsaH I, Hae II, Hha I, Nar I, Nla IVBsaW 1 (W/CCGGW)Age I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)Age I, BsaW I, BsrF I, Hpa I

10、IAva I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBspEIBsaW I, BspE I, Hpa IIBsrF I (R/CCGGY), NgoM IVBsrF I, Hpa IINgoM IVHpa IIBsiE 1(CGAT/CG)PadMsel(CGAT/CG)Pvu IBsiE I, Dpn II, Pvu I(CGGC/CG)Sac IIAci IBsiHKA 1 (GTGCA/C)Bsp1286 I (GTGCA/C)BsiHKA I, Bsp1286lBsp1286 I (GAGCA/C)BsiHKAI, Bsp1286lBsp1286 I

11、 (GAGCT/C), Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac INsi IPst I, Sbf IBsg IBsiW 1 (C/GTACG)Acc65 I, Ban I (G/GTACC), BsrG IRsa IBsp1286 I(GGGCC/C)Apa I, Ban II (GGGCC/C)Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I(GTGCA/C)BsiHKA IApaL I, BsiHKA I, Bsp1286l(GGGCC/C)Ban II (GGGCC/C)Ban II,

12、Bsp1286 I(GAGCT/C)Ban II (GAGCT/C), BsiHKA I, Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac I(GWGCW/C)BsiHKA IBsiHKA I, Bsp1286l(GTGCA/C)Nsi I-(GTGCA/C)Pst I, Sbf IBsg IBspE IAge I, BsaW I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)BsaW I, Hpa II(T/CCGGA)Ava 1 (C/CCGGG), Xma 1Hpa II, Nci I, ScrF IBsaW 1Bsa

13、W I, BspE I, Hpa IIBsrF 1 (R/CCGGY), NgoM IVHpa IIBspH 1 (T/CATGA)Afl III (A/CATGT), Neo 1, Sty 1 (C/CATGG)NlalllBsrFI(A/CCGGY)Age 1, BsaW 1Age I, BsaW I, BsrF I, Hpa II(G/CCGGY)Age 1, BsaW 1, NgoM IVBsrF I, Hpa II(R/CCGGY)Ava 1 (C/CCGGG), Xma 1Hpa II, Nci I, ScrF I(A/CCGGY)BsaW 1, BspE 1BsaW I, Hpa

14、 II(R/CCGGY)BsaW 1, BspE 1Hpa II(G/CCGGY)NgoM IVBsrF I, Cac8 I, Hpa II, Nae I(CR/CCGG YG)SgrAIBsrF I, Hpa IIBsrG 1 (T/GTACA)Acc65 1, Ban 1 (G/GTACC), BsiW 1Rsa IBssH IIAfl III (A/CGCGT), Mlu 1BstU I, Hha I(G/CGCGC)Asc 1BssH II, BstU I, Cac8 I, Hha IBstBIAcc 1 (GT/CGAC), Cla 1, Taq 1Taq I(T T/CGAA)Ac

15、il, Acl 1, BsaH 1 (GR/CGYC), HinP1 I, Hpa II, Nar IBstYI(A/GATCY)BamH I, Bgl IIAlw I, BstY I, Dpn II(G/GATCY)BamH 1Alw I, BamH I, BstY I, Dpn II, Nla IV(R/GATCY)Bel 1, Dpn IIDpn II(G/GATCY)Bel 1, Dpn IIAlw I, Dpn II(A/GATCY)Bgl IIBgl II, BstY I, Dpn IIClal (AT/CGAT)Acc 1 (GT/CGAC), BstB 1, Taq 1Taq

16、IAcil, Acl 1, BsaH 1 (GR/CGYC), HinP1 I, Hpa II, Nar I-Dpn ll/Mbo 1/Sau3A 1 (/GATC)BamH I, BstY I (R/GATCC)Alw I, Dpn IIBel I, Bgl II, BstY I (R/GATCY)Dpn IIEael(Y/GGCCR)PspOM IHae III, Sau96 I(C/GGCCR)EaglBsiE I, Eae I, Eag I, Hae III(T/GGCCR)EaglEae I, Hae III(C/GGCCR)Not IAci I, BsiE I, Eae I, Ea

17、g I, Fnu4H I, Hae III(T/GGCCR)Not IAci I, Eae I, Fnu4H I, Hae IIIEagl (C/GGCCG)PspOM IHae III, Sau96 IEae I (Y/GGCCR)Eae I, Hae IIIEae I (C/GGCCG)BsiE I, Eae I, Eag I, Hae IIINot IAci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae IIIEcoRI(G/AATTC)Apo I (G/AATTC)Apo I, EcoR I, Tsp509 IApo I (R/AATTY)Apo I, T

18、sp509 IMfe I, Tsp509 ITsp509 IHinP1 I(G/CGC)Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq IAcil, BsaH I (GR/CGCC), Nar IHhalBsaH I (GR/CGTC)Hga IHpa IIAci IHpa ll/Msp I(C/CGG)Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I-Aci I, BsaH I (GR/CGCC), HinP1 I, Nar IAci IKas I(G/GCGCC)Ban I (G/GCGCC)Ban I, Bsa

19、H I, Hae II, Hha I, Kas I, Nar I, Nla IVMfel (C/AATTG)Apo I (R/ATTTY), EcoR I, Tsp509 ITsp509 IMlu I(A/CGCGT)Afl III (A/CGCGT)Afl III, BstU I, Mlu IAsc I, BssH IIBstU I, Hha IMsel(T/TAA)Ase IMselBfa I, Csp6 I, Nde INar I(GG/CGCC)Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I-Aci I, HinP1 IHhalBsaH I (

20、GR/CGCC)Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IVBsaH I (GR/CGTC)BsaH I, Hga IHpa IIAci INeo IAfl III (A/CATGT), BspH INla III(C/CATGG)Sty 1 (C/CATGG)BsaJ I, Neo I, Nla III, Sty IPcilNlalllNdel(C/VTATG)Ase 1, Bfa 1, Csp6 1, Mse 1NgoM IV (G/CCGGC)Age 1, BsaW 1, BsrF 1 (R/CCGGY), SgrA 1BsrF I, Hpa I

21、IAva 1 (C/CCGGG), Xma 1Hpa IL Nci I, ScrF IBsaW 1, BspE 1Hpa IIBsrF 1 (R/CCGGC), SgrA 1BsrF I, Cac8 I, Hpa II, Nae INhel(G/CTAGC)Avr II, Spe 1, Sty 1 (C/CTAGG), Xba 1Bfa INlalll(CATG/)Sph 1, Nsp 1NlalllNot 1(GC/GGCCGC)PspOM 1Aci I, Eae I, Fnu4H I, Hae IIIEaglAci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae

22、 IIIEae 1 (Y/GGCCR)Aci I, BsiE I, Eae I, Fnu4H I, Hae IIINsil (ATGCA/T)BsiHKA 1 (GTGCA/C), Bsp1286 I (GTGCA/C), Pst I, Sbf INsp 1 (RCATG/Y)Nla III, Sph INlalllPac 1(TTAAT/TAA)BsiE I (CGAT/CG), Pvu IMse IPci 1 (A/CATGT)Afl II (A/CATGT), BspH 1, Neo 1, Sty 1 (C/CTAGG)NlalllPpuM 1(RG/GWCCY)Ava II, Rsr

23、IIAva ll9 Sau96 I(GG/GTCCY)Ava II, Rsr IIAva II, BsmF I, Nla IV, Sau96 I(GG/GACCY)Ava II, Rsr IIAva II, Nla IV, Sau96 IPspOM 1 (G/GGCCC)Eae 1 (Y/GGCCR), Eag 1Hae III, Sau96 INot 1Aci I, Fnu4H I, Hae III, Sau96 IPstl(CTGCA/G)BsiHKA 1, Bsp1286 I (GTGCA/C)Bsg INsilSbf IPstlPvu 1 (CGAT/CG)AsiSIDpn I, Pv

24、u IPadMselBsiE I (CGAT/CG)BsiE I, Dpn II, Pvu IRsrll(CG/GWCCG)Ava II, PpuM I (RG/GACCY)Ava II, Sau96 IPpuM I (RG/GACCY)Ava II, Nla IV, Sau96 IPpuM I (RG/GTCCY)Ava II, BsmF I, Nla IV, Sau96 ISac 1 (GAGCT/C)Ban II (GAGCT/C), BsiHKA I, Bsp1286 I (GAGCT/C)Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac ISac II

25、(CCGC/GG)BsiE I (CGGC/CG)Aci ISall(G/TCGAC)Ava 1 (C/TCGAG), Xho 1Taq ISbf 1 (CCTGCA/GG)BsiHKA 1, Bsp1286 I (GTGCA/C)Bsg INsi 1-PstlPstlSfcl(C/TGCAG)ApaL 1Bsg ISgrAI (CR/CCGG YG)See BsrF 1Spe 1 (A/CTAGT)Avr II, Nhe 1, Sty 1 (C/CTAGG), Xba 1BfalSph 1 (GCATG/C)Nla III, Nsp 1Nla IIISty 1AvrilAvr II, Bfa

26、 I, BsaJ I, Sty I(C/CTAGG)Nhe 1, Spe 1, Xba 1Bfal(C/CATGG)BspH 1Nla IIINeo 1BsaJ I, Neo I, Nla III, Sty ITaq 1 (T/CGA)Acc 1 (GT/CGAC), Cla 1, BstB 1Taq IAcil, Acl 1, BsaH 1 (GR/CGYC), HinP1 l,Hpa II, Nar ITsp509 1 (/AATT)Apo I (R/AATTY), EcoR I, Mfe ITsp509 IXbal (T/CTAGA)Avr II, Nhe 1, Spe 1, Sty 1 (C/CTAGG)BfalXho l/TlilAva 1 (C/TCGAG)Ava 1, Taq 1, Xho 1(C/TCGAGSallTaq 1Xma 1Age 1, BsaW 1, BspE 1, BsrF 1, NgoM IV, SgrA 1Hpa II, Nci 1, ScrF 1(C/CCGGGAva 1 (C/CCGGG)Ava 1, BsaJ 1, Hpa II, Nci 1, ScrF 1, Sma 1, Xma 1