8 常用载体通用引物.docx

1、8 常用载体通用引物载体名称上游引物（5primer）下游引物（3primer）P3*FLAG-CMV CMV-30(825-854) CMV-24(1129-1152)pAc5.1/V5-His(_A,_B,_C)pAc5-5BGH revpAcG2TpGEX-5not availablepACT T7EEVT3/EBVrevpACT2 GAL4 ADforpGAL4-ADrvpACT2p17110p12584pACYC184(BamHI-site) PBRforBamPBRrevBampACYCDuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1pACYCDue

2、t-1(MCSII) DuetUp2T7-TerminatorpAdEasy-1not availablenot availablepAdTrack-CMVnot availablenot availablepAS2-1 GAL4 Bdfor/P24990pGAL4-BDrv/P31430pB42AD pB42ADF PB42ADRpBABE pBMN_5pBacPAK8 BAC1 BAC2pBADpBAD-FpBAD-RpBAD/HisABCpBAD-5pBBR1MCST3/M13revT7/M13forpBD-GAL4 pBD-GAL4-F PBD-GAL4-RpBI121 35S NOS

3、pBINDGAL4-BdforT3,EBVrevpBK-CMV M13F/T7 M13R/T3pBluescriptIIKS(+/-)T3/M13revT7/M13for（优先用M13F）pBluescriptIISK(+/-)T3/M13revT7/M13forpBluescriptKS(+/-)T3/M13revT7/M13forpBluescriptSK(+/-)T3/M13revT7/M13forpBR322(BamHI-site) PBRforBamPBRrevBampBR322(EcoRI/HindIII-sites) pBRforEcopBRrevHindpBR325(BamHI

4、-site) PBRforBamPBRrevBampBR329(BamHI-site) PBRforBamPBRrevBampBridge(MCSI)GAL4-Bdfor3BDpBridge(MCSII)not availablenot availablepBS-T IIT3/M13revT7/M13forpBS（AMP）T7T3pBSR T3 T7terpBudCE4.1(MCSI)CMV-Profor,T7c-mycrev,EBVrevpBudCE4.1(MCSII)EF-1 FwdBGHpBudCE4.1/lacZ/CATCMV-Profor,T7c-mycrev,EBVrevpBV22

5、0 pBV220F PBV220RpCAL-cT7/Tac-ProforT7-TerminatorpCAL-nT7/Tac-ProforT7-TerminatorpCAMBIA1301(1300)pCAMBIA1301-5(ECORI)/M13RpCAMBIA1301-3(HindIII)pCAMBIA1304pCAMBIA1301-5(ECORI)pCAMBIA1301-3(HindIII)pCAMBIA2300 M13R(-48) M13F(-47)/M13F(-49)pCANTB5E S1 S6pCAT3-enhancer RVP3 此载体无反向引物pCDFDuet-1(MCSI)ACY

6、CDuetUP1 Primer DuetDOWN1pCDFDuet-1(MCSII) DuetUP2 T7TERpcDNA1.1CMV-Profor,T7 pCDM8-3pcDNA2.1M13for/T7 M13rev/Tac-ProforpcDNA3T7/CMV-ProforBGH rev/SP6pcDNA3.1(+/-)pcDNA3.1F/T7/CMV-ProforBGH revpcDNA3.1/His(_A,_B,_C)T7/CMV-ProforBGH revpcDNA3.1/myc-His(_A,_B,_C)T7/CMV-ProforBGH rev/c-mycrevpcDNA3.1/V

7、5-His(_A,_B,_C)T7/CMV-ProforBGH rev/V5revpcDNA3.1/Hygro(+,-)T7/CMV-ProforBGH rev/V5revpcDNA3.1/Zeo(+.-) T7BGHrevpcDNA3.3TOPOTACMV-ProforTKPAreversepcDNA4/HisMax(_A,_B,_C) XpressforBGH revpcDNA4/HisMax-TOPO XpressforBGH revpcDNA4/HisMax-TOPO/lacZ XpressforBGH revpcDNA4/myc-His(_A,_B,_C) T7,CMV-Profor

8、BGH rev,c-mycrevpcDNA4/TOTREfor/CMV-ProforBGH revpcDNA4/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5revpcDNA4-TET/ON/AMP+CMV-ProforpcDNA5/FRTT7/CMV-ProforBGH revpcDNA6/myc-His(_A,_B,_C)CMV-Profor,T7BGH rev,c-mycrevpcDNA6/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5revpCEP4 pCEP-F/CMV-ProforEBVrevpCIT7（17BASE）

9、EBVrevpCI-neoT7（17BASE）EBVrev,T3pCMS-EGFP T7 T3 pCMS-EGFPT7EBVrev,T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7pCMV5 pCMV5F PCMV5RpCMV6CMV-ProforpCMV6-3、HGHrevpCMV6-XL4CMV-Profor/T7pCMV6-3、HGHrevpCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R，EBVrevpCMV-MYC(AMP+) PCMV-F PCMV-R,EBVrevpCMVmyc/nucCMV-ProforBGH rev,c-mycre

10、vpcmv-scriptEXVectorT3T7pCMV-Sport6T7/M13forSP6/M13revpCOLADuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1pCOLADuet-1(MCSII) DuetUP2 T7TERpCR2.1T7,M13forM13revpCR2.1-TOPOM13for,T7M13revpCR3.1T7BGH revpCR3.1-UniT7BGH revpCR4T3/M13revT7/M13forpCR4-BluntT3/M13revT7/M13forpCR4-TOPOM13rev,T3M13for,T7pCR-BluntM1

11、3revT7,M13forpCR-BluntIIM13rev/SP6T7/M13forpCR-BluntII-TOPOM13rev/SP6M13for/T7pCRIIM13rev/SP6T7/M13forpCRII-TOPOM13rev/SP6M13for/T7pCR-ScriptSK(+)T7/M13forT3/M13revpCR-XL-TOPOM13revT7,M13forpCS2+(MCSI)SP6 EBVrev,T7?pCS2+(MCSII)T3pSG5-3pDB_GAL4camT7PpDB_leuPDBLeu-5PDBLeu-3pDEST22DEST22FDEST22RpDEST15

12、pGEX-5T7-TerminatorpDEST20pGEX-5pFastBac-3pDEST26TREfor/CMV-ProforT7/M13forpDisplayT7/CMV-Proforc-mycrevpDNR-LIB(MCS_A)M13for,T7pSG5-3,M13RpDonar M13F M13RpDONR201 PDONR-F PDONR-RpDONR207 PDONR-F PDONR-RpDONR223 M13F M13R pDriveT7/M13revSP6/M13forpDsRED1-C1 (DsRed1-N-f) DsRed1-C-rpDsRed1-N1CMV-Profo

13、rRFP-Nrev,CMV-R?pDsRed2-N1 (DsRed1-N-f) DsRed1-C-rpDsRed2-C1 (DsRed1-N-f) DsRed1-C-rpDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3pDsRed-Express dsRed1_N_primer/CMV-F dsRed1_C_primer/CMV-RpDSRED-monomer-N1(KAN+) dsRed1_N_primerpDSRED-N1(KAN+) pEGFP-N-5 pDSRED-N-R pEASY-Blunt M13FT7/ M13RpEASY-BluntSimple

14、 M13FT7/ M13R（距离插入位点合适，优先安排）pEASY-T1 Simple M13FM13R（距离插入位点合适，优先安排）pEASY-T1 M13F/T7 M13RpEASY-T3 M13F/T7 M13R/SP6pECFP-N(C) PECFP-N-5. PECFP-N-3,pEF/myc/cyto/ER/mito/nuc pEF-5 Pcdna3.1R/BGH revpEF1(4,6/myc-His)T7/pEF-5 Pcdna3.1R/BGH revpEF1(4,6/V5-His)T7/pEF-5 Pcdna3.1R/BGH revpEF1(4;6/His_A;_B;_C)T

15、7/Xpressfor Pcdna3.1R/BGH revpEF5/FRT/V5-D(-TOPO)T7/pEF-5 Pcdna3.1R/BGH revpEGFPM13revEGFP-NrevpEGFP-1not availableEGFP-Nrev/ PEGFP-N-3pEGFP-C PEGFP-C-5 PEGFP-C-3pEGFP-C1EGFP-CforSV40-pArevpEGFP-C2EGFP-CforSV40-pArevpEGFP-C3EGFP-CforSV40-pArevpEGFP-FEGFP-CforSV40-pArevpEGFP-N PEGFP-N-5 PEGFP-N-3pEGF

16、P-N1CMV-ProforEGFP-NrevpEGFP-N2CMV-ProforEGFP-NrevpEGFP-N3CMV-ProforEGFP-NrevpENTRM13FM13RpET-11(-a,-b,-c,-d)T7T7-TerminatorpET-12(-a,-b,-c)T7T7-TerminatorpET-14bT7T7-TerminatorpET-15bT7T7-TerminatorpET-16bT7T7-TerminatorpET-17bT7T7-TerminatorpET-17xbT7T7-TerminatorpET-19bT7T7-TerminatorpET-20b(+)T7

17、T7-TerminatorpET-21(-a,-b,-c,-d)(+)T7T7-TerminatorpET-22b(+)T7T7-TerminatorpET-23(-a,-b,-c,-d)(+)T7T7-TerminatorpET-24(-a,-b,-c,-d)(+)T7T7-TerminatorpET-25b(+)T7T7-TerminatorpET-26b(+)T7T7-TerminatorpET-27b(+)T7T7-TerminatorpET-28a(-b,-c)(+)T7T7-TerminatorpET-29a(-b,-c)(+) S.tag/T7T7-TerminatorpET-3

18、(-a,-b,-c,-d)T7T7-TerminatorpET-30_Ek/LICT7T7-TerminatorpET-30_Xa/LICT7T7-TerminatorpET-30a(-b,-c)(+) S.tag/T7T7-TerminatorpET-31b(+)not availableT7-TerminatorpET-32_Ek/LICTRXforT7-TerminatorpET-32_XA/LICTRXforT7-TerminatorpET-32a(-b,-c)(+) S.tag/TRXforT7-TerminatorpET-33b(+)T7T7-TerminatorpET-34b(+

19、) S.tagT7-TerminatorpET-35b(+) S.tagT7-TerminatorpET-36b(+) S.tagT7-TerminatorpET-37b(+) S.tagT7-TerminatorpET-38b(+)not availableT7-TerminatorpET-39b(+) S.tagT7-TerminatorpET-40b(+) S.tagT7-TerminatorpET-41_Ek/LICGST-CforT7-TerminatorpET-41a(-b,-c)(+)S.tag/pGEX-5T7-TerminatorpET-42a(-b,-c)(+) S.tag

20、/pGEX-5T7-TerminatorpET-43.1a(-b,-c)(+) S.tagcoliDOWNpET-43.1a_EK/LIC S.tagnot availablepET-44a(-b,-c)(+) S.tagT7-TerminatorpET-44a_EK/LIC S.tagnot availablepET-45b(+)T7T7-TerminatorpET-46_Ek/LICT7T7-TerminatorpET-5(-a,-b,-c)T7T7-TerminatorpET-52b(+)T7T7-TerminatorpET-7T7T7-TerminatorpET-9(-a,-b,-c,

21、-d)T7T7-TerminatorpETcoco-1T7T7-TerminatorpETDuet-1(MCSI) pETUP1 DuetDOWN1pETDuet-1(MCSII) DuetUp2 T7-TerminatorpET-T4T7T7-TerminatorpEYFP-C1 pEGFP-C-5 pEGFP-C-3 pEYFP-N1 pEGFP-N-5,CMV-Profor pEGFP-N-3 pFLAG-ATSN26for pTrcHis-rv；C24revpFLAG-CTCN26for pTrcHis-rv；C24revpFLAG1N26for pTrcHis-rv；C24revpF

22、LAG-CMV(-2) CMV-30；CMV-ProforCMV-24pFLAG-CMV1 CMV-30CMV-24pGAD424 GAL4_ADfor 3ADpGADGH GAL4_ADfor 3ADpGADT7 T7/GAL4_ADfor 3ADpGADT7-Rec T7/GAL4_ADfor 3AD pGADT-T T7 3,BDpGAD10GAL4_ADfor 3AD pGAPZa-A a-FACTOR 3AOX pGBKT7 T7 3,BDpGBT9MATCHMAKER5DNA-BD/GAL4BDMATCHMAKER3DNA-BDpGEM-1T7SP6pGEM-3T7SP6pGEM-

23、3Zf(+)(-)SP6/M13revT7/M13forpGEM-4T7SP6pGEM-4ZT7/M13forSP6/M13revpGEM-5Zf(+)(-)SP6/M13revT7/M13forpGEM-7Zf(+)(-)SP6/M13revT7/M13forpGEM-9Zf(+)(-)T7/M13forSP6/M13revpGEM-TT7/M13forSP6/M13revpGEM-T_EasyT7/M13forSP6/M13revpGene/V5-His_A(_B,_C)pGENE-5BGH rev,V5revpGEX-2TpGEX-5pGEX-3pGEX-2TKpGEX-5pGEX-3p

24、GEX-3XpGEX-5pGEX-3pGEX-4T-1(-2,-3)pGEX-5pGEX-3pGEX-5X-1(-2,-3)pGEX-5pGEX-3pGEX-6P-1(-2,-3)pGEX-5pGEX-3pGL2 GLP1 GLP2pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2)pGL3-Basic(MCSI)RVP3(PGL3+)?GLP2(PGL3-)?pGL3-Basic(MCSII)RVP4,PLXSN-3?pGL4pGlow(-TOPO)T7pGlow-3pGM-T T7 SP6pGWC SP6 T7pHook-2T7/CMV-Profornot available

25、pIND/V5-His_A(_B,_C)not availableBGH rev,V5revPinpointTMVector PinpointPrimer SP6pIRES(MCSI)T7pIRES-3pIRES(MCSII)not availableT3,EBVrevpIRES2-EGFPCMV-Profor,pIRES2-EGFP.P5 IRESrev,pIRES2-EGFP.P3pIRES-hrGFP-2aCMV-Profor,T3pIRES-hrGFP-3pIRESneo2T7/CMV-ProforpIRES-3pIVEX(all)T7/pBRrevBamT7-TerminatorpL

26、EXA pLEXA-F pLEXA-R pLNCX pLNCX-F pLNCX-RpLNCX2 CMV-ProforpRevTRE-3pLVTHM（H1启动子下游）H1primerSP6pLXSN pLXSN-F pLXSN-R pMALc MalE primer M13F(-47) pMAL-c2E MalE primer M13F(-47)pMAL-C2x Pmal-c2x-F(P5) Pmal-C2X-R(P3 )pMALd MalE primer M13F(-47) pMALe MalE primer M13F(-47) pMAL-P4X MalE primer M13F(-47) pMAL-p2X MalE primer M13F(-47) pMET A/B/C AUG1F AUG1RpMD18-T M13R(-48)ECORI M13F(-47)(HIND)pMD19-T M13F47 M13R48pMD20-T M13F47 M13R48pMOSBlueT7/M13revM13forpMSCV-puro pLXSNfw