常用荧光染料的激发及发射波长.docx

1、常用荧光染料的激发及发射波长荧光素名称激发波长 (nm)发射波长 (nm)备注1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM (5-Carboxyfluoresce

2、in)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)578567 6045915-TAMRA (5-Carboxytetramethylrhodamine)548542 5525686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hy

3、droxy-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471, 474ABQ344445Acid Fuchsin540630ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine

4、 Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFPAlexa Fluor 350346342 442441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531, 532553, 554Alexa Fluor 5

5、46556, 557572, 573Alexa Fluor 568577, 578603Alexa Fluor 594590, 594617, 618Alexa Fluor 633632650Alexa Fluor 647647666Alexa Fluor 660668698Alexa Fluor 680679702Alizarin Complexon530-560, 580580, 624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumar

6、in)345347 425444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346350 442445Aminomethylcoumarin (AMCA)345347 425444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+APTS424505Astrazon Brilliant Re

7、d 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG CBQCA465560ATTO-TAG FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9 (Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine Sulphate4305

8、50Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)Blue Fluorescent Protein381, 382, 383445, 447, 448blue shifted GFP (Y66H)Blue Fluorescent ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Blancophor FFG390470Blanc

9、ophor SV370435BOBO -1462481BOBO -3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/67660

10、5676Bodipy Fl504, 505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO -1462481BO-PRO -3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/4015

11、33/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crimson588, 589611, 615Calcium Green501, 506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calcium

12、 Orange549575576Calcofluor White385, 395, 405437, 440, 445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue377398399 420423Cascade Yellow399400 550552Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430, 433, 436, (453)474, 475, 476, (501)Cyan Fluorescent ProteinCFP/YFP FRETCh

13、lorophyll480650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Coelenterazine Ca2+ Dye, bioluminescence(429)465Ca2+ Dye, bioluminescence, native moleculeCoelenterazine cp (Ca2+ Dye,)(430)442Ca2+ Dye, bioluminescenceCoelenterazine f(437)473Ca2+ Dye,

14、bioluminescenceCoelenterazine fcp452Ca2+ Dye, bioluminescenceCoelenterazine h(437)464Ca2+ Dye, bioluminescenceCoelenterazine hcp(433)444Ca2+ Dye, bioluminescenceCoelenterazine ip441Ca2+ Dye, bioluminescenceCoelenterazine n(431)467Ca2+ Dye, bioluminescenceCoelenterazine O460575Coumarin Phalloidin3874

15、70C-phycocyanineCPM Methylcoumarin384469MethylcoumarinCTC400-450602CTC FormazanCy2489506Cy3.1 8554568Cy3.5581598Cy3514552554 566570Cy5.1 8649666Cy5.5675695Cy5649666670Cy7710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520Cell Prolife

16、ration AssayDabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH (Dichlorodihydrofluorescein Diacetate)505535DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS4

17、96705Di-8-ANEPPS (non-ratio)488498 605713DiA (4-Di-16-ASP)456591Dichlorodihydrofluorescein Diacetate (DCFH)505535DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3)549, 551565Dinitrophenol349DiO (DiOC18(3)484, 487501, 502DiR748780

18、Lipophilic TracerDiR (DiIC18(7)750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent proteinDTAF494520DY-630-NHS621660Hemicyanelabel for proteins and DNADY-635-NHS634664Hemicyanelabel for proteins and DNAEBFP383447Enhanced Blue Fluorescent ProteinECFP436474

19、Enhanced Cyan Fluorescent ProteinEGFP488, 498507, 516Enhanced Green Fluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchrysin430540EukoLightEuropium (III) chlorideEYFP513, 520527, 532Enhanced

20、Yellow Fluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480 506, 506520, 527Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald49

21、5524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red (high pH)572657Fura Red/Fluo-3Fura-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF4

22、30485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoec

23、hst 33342347483Hoechst 34580392440HPTS355465Hydroxycoumarin325-360386-455Hydroxystilbamidine (FluoroGold)361536Hydroxytryptamine400530Indo-1, high calcium330401Emission ratio dyeIndo-1, low calcium346475Emission ratio dyeIndodicarbocyanine (DiD)644665Indotricarbocyanine (DiR)748780Intrawhite Cf36043

24、0JC-1514529JO-JO-1530545JO-PRO-1532544LaserPro795812Laurodan355460LDS 751 (DNA)543712LDS 751 (RNA)590607Leucophor PAF370430Leucophor SF380465Leucophor WS395465Lissamine Rhodamine572, 577591, 592Lissamine Rhodamine B577592LIVE/DEAD Kit Animal CellsCalcein/Ethidium homodimer494528517617for more details referto LOLO-1566580LO-PRO-1568581Lucifer Yellow425, 428528, 536, 540Lyso Tracker Blue373422Lyso Tracker Blue-White466536Lyso Tracker Green504, 534511