微流控芯片之一.pptx

微流控芯片之一.pptx

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LabonaChip,Or:

TheIncredibleShrinkingScienceExperiment!

Or:

APOIBE!

Credits:

MathiesLab,UC-BerkeleyQuakeLab,StanfordAgilent,Inc.,二十一世纪科学发展趋势,MiniaturizationAutomationIntegrationPortable,LabonaChip?

Microfluidics,Microfluidics:

TheuseofmicrofabricationtechniquesfromtheICindustrytofabricatechannels,chambers,reactors,andactivecomponentsonthesizescaleofthewidthofahumanhairorsmaller,Credit:

Dr.KarenCheung,UBCECE,Whyusemicrofluidics?

Credit:

Dr.KarenCheung,UBCECE1moleculein1mL=1.6x10-18M1moleculein1nL=1.6x10-15M1moleculein1pL=1.6x10-12M,SamplesavingsnLofenzyme,notmLFasteranalysescanheat,coolsmallvolumesquicklyIntegrationcombinelotsofstepsontoasingledeviceNovelphysicsdiffusion,surfacetension,andsurfaceeffectsdominateThiscanactuallyleadtofasterreactions!

Whyusemicrofluidics?

1-3min/12samples,Microchipelectrophoresis1-3minMulti-channelmicrochipelectrophoresis,Highvoltage,buffer,sample,capillary,10-30min,Capillaryelectrophoresisseparationdetection,buffer,1-3,Gelelectrophoresis,SomeinterestingLOCapplications,GeneticanalysisPortabledevicesforpathogendetectionHigh-throughputbiologyChemicalsynthesisCellImmunoassayanalysisMetaboliteanalysisDrugdeliverysystemsSinglemolecularEnzymeanalysis,CourtesyofDr.BorisStoeber,Geneticanalysismicrosystems,96-channelradialcapillaryarrayelectrophoresismicroplate,AnalChem.1999;71（23）:

5354-61,Foodpoisoning,BacterialPathogensWoundinfections,Biowarfare,Foodpoisoning:

PathogenicE.coli:

1in10,000inCanadaSalmonellaspp.:

ashighas1in50eggs,Woundinfections:

Antibiotic-resistantS.aureus:

ashighas50%LungDisease:

Pseudomonasspp.MycobacteriumtuberculosisBiowarfare:

Anthrax,plague,smallpox,LungDisease,Dr.SharonPeacock,UniversityofOxford,MethodsforGeneticDetectionofPathogens,OLDWAY:

NEWWAY:

Developbacterialpathogendetectorsthatare:

Rapid（1hour）General（manydifferenttypesofpathogens）Sensitive（detectasinglecell）GeneticallySpecificField-portable,PCR-CEMicrosystemFabrication,PortableSystemforInfectiousDiseaseDetection,Systemmeasures8x10x12inches,Completefluorescencedetectionincluding488-nmsolid-statelaser,PMT,optics,anddetectionelectronicsHeaterandRTDelectronics4high-voltageCEpowersuppliesPDMSmicrovalvehardwareSimplePCinterface,Time（seconds）,ratureC）,ThermalCyclingCharacteristics,Time（seconds）5Xfasterthanconventionalthermalcyclers,Application:

Tuberculosis（TB）detection,DiseasecausedbyMycobacteriumtuberculosis9millionnewcasesofactiveTBeveryyearworldwide2millionTBdeathseveryyear450,000newcasesofmulti-drugresistantTB/yr10%ofTBcasesinSouthAfricaareXDRTBstrains,resistanttofirst-andsecond-lineantibioticsDoctorsWithoutBorders:

CurrentTBdetectionmethods,Fixationandstainingwithacid-faststains（Ziehl-Neelsenorauraminestains）Opticalorfluorescencemicroscopeexamination（10,000organisms/mL）Cultureinbiosafetylevel3containmentfacilityInselectcases,moleculardetectionisusedtoverifydiagnosisThatTBdestroysmillionsoflivesaroundtheworldeveryyearshowsthatthecurrentapproachisjustnotworkingThetoolswehavetotreatanddiagnoseTBarewoefullyinadequateandoutdated,andwerenotseeingthenecessaryurgencytotacklethedisease.Dr.TidovonSchoen-Angerer,Director,DWBsCampaignfortheAccesstoEssentialMedicines,TheNot-so-DistantFuture,2008PDA2308?

Paramount,384根通道扫描检测,Emrich,C.A.,etal.,AnalyticalChemistry,2002.74（19）:

p.5076-5083.,10根通道CCD同时检测,Dang,F.Q.,etal.,AnalyticalChemistry,2005.77（7）:

p.2140-2146.,目前最高通量微流控芯片平台,Aborn,J.H.,etal.,LabonaChip,2005.5（6）:

p.669-674,门控进样多通道检测,Xu,X.M.,etal.,AnalyticalChemistry,2002.74（21）:

p.5517-5522,高通量均相免疫分析,Bromberg,A.andR.A.Mathies,Electrophoresis,2004.25（12）:

p.1895-1900,Microbes,cellsandsmallorganismscomeinawidearrayofshapesandsizesYeast,BloodCells,cryptosporidium,Lab-on-a-chipDevicesforCellSeparationandIdentification,MicrofluidicorLab-on-a-chipDevicescanbeusedtoseparatedifferentcelltypes,Variousbloodcellseparationshavebeendemonstrated:

J.A.Davis,D.W.Inglis,K.M.Morton,D.A.Lawrence,L.R.Huang,S.Y.Chou,J.C.Sturm,andR.H.Austin,Deterministichydrodynamics:

takingbloodapart,Proc.Nat.Acad.Sci.（USA）,vol.103,pp.14779-14784,2006.,D.W.Inglis,J.A.Davis,T.J.Zieziulewicz,D.A.Lawrence,R.H.Austin,andJ.C.Sturm,Determiningbloodcellsizeusingmicrouidichydrodynamics,J.Immunol.Meth.,vol.329,pp.151-156,2008.D.W.Inglis,K.J.Morton,J.A.Davis,T.J.Zieziulewicz,D.A.Lawrence,R.H.Austin,andJ.C.Sturm,Microfluidicdeviceforlabel-freemeasurementofplateletactivation,SubmittedtoLabonaChip,Jan08.Butnoworkyetwithmicroorganisms!

Thisprojectwillleadtopublicationswhichdemonstratetheseparationofmicroorganismsonportableinexpensivepolymermicrofluidicdevices.,Suchdevicesmayhaveapplicationsinearlydetectionofhumandiseasessuchassepsisandvariousparasiticdiseasesaswellasmanagementofswimmingpoolanddrinkingwaterquality.,WhatisMicro-Fluidics,MicrofluidicsmicrofabricatedstructuresforfluidhandlingEngineersknowhowtomicrofabricateandbuildintegratedsystems,mTASMicroTotalAnalysisSystemIntegratemicrofluidiccomponentsintoausefuldevice,a“LabonaChip”,“developmentandapplicationofmicro-andnanofabricateddevicesandsystemsforchemicalandbiochemicalmeasurements.Thetechnologyencompassesextensiveareasofapplicationinclinicaldiagnostics,genomicsandproteomics,environmentalassays,separationscience,cellularanalysisordrugdiscovery.”FromM,InterdisciplinaryPhysics,Chemistry,Biology,Photolithography,ReactiveIonEtching,Drillaccessholes,Sealwithcoverslip,FabricationProcess:

Fluidgoesin,1.4mm,1.5mm,1.6mm,1.7mm,1.8mm,1.9mm,mmmmmm,Peakwidth10nmNoknownmethodfractionateswithsuchresolution.,HighResolutionSeparationwithSize-PositionCorrelation,Canincludechirptofractionateoverarangeofsizesbyvaryinggor,Huangetal,Science2004,CellSeparationbySize,Whitebloodcellscomeindifferentsizesandcanbedifferentiated,HealthyLymphocytes,CancerousLymphocytes,Humanplateletschangesizeinresponsetochemicalandtemperaturestimulus,