Cazymes对比分析流程.docx
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Cazymes对比分析流程
Cazymes对比分析流程
NCBI数据库地址
以Fibrobactersuccinogenes为例。
1.进入NCBI数据库，输入Fibrobactersuccinogenes，在Gene下查找
，
图1
进入下面的界面，
图2
以FSU_2293为例,基因注释为木聚糖酶，点击进去，
图3
发现FSU_2293，属于GH10，有CBM6，将此基因进行统计，见下表。
GH10
FSU_2293
CBM6
BTD
xylanase
xylanase
若是NCBI上没有显示相关的GH家族或CBM，则可以进行BLAST。
点击图3中的“FASTA”，复制相关基因进行BLAST。
例如CHU_0868，是糖基转移酶相关的基因，但是没有显示具体的GT家族。
图4
进行BLAST，
图5
点击相关的结构域，进入另一个网页，
图6
可以看到，此基因属于GT1家族。
或是进行Pfam结构域搜索：
网址：
，
输入相关基因，点击
，
图7
可以看到，此基因具有GT1结构域，属于GT1家族。
分别统计glycosidehydrolase（GH）、glycosyltransferase（GT）、 polysaccharidelyase（PL）、carbohydrateEsterase（CE）。
对于glycosidehydrolase可以统计与纤维素降解和半纤维素降解相关的基因。
格式见下表。
注意：
表中标红的列为必填项目，“CharacterizedActivity”为图2中的“Descriprition”。
CAZy
Family
MYPLocus
CBMFamily
InSilicoPrediction
CharacterizedActivity
GH（74）
GH
MYP_0567
hypotheticalprotein
GH
MYP_0581
hypotheticalprotein
GH
MYP_0913
hypotheticalprotein
GH
MYP_1026
hypotheticalprotein
GH
MYP_1096
hypotheticalprotein
GH
MYP_3811
endoglucanase
endoglucanase
GH
MYP_4017
xyloglucanase
xyloglucanase
GH
MYP_4345
CBM9
beta-xylosidase/endoglucanase
beta-xylosidase/endoglucanase
GH
MYP_4348
CBM4_9
glycerolkinase
glycerolkinase
GH1
MYP_2015
beta-galactosidase
beta-galactosidase
GH2
MYP_3616
beta-mannosidase
beta-mannosidase
GH3
MYP_0984
b-glucosidase
b-glucosidase
GH3
MYP_1602
hypotheticalprotein
GH3
MYP_1909
b-glucosidase
b-glucosidase
GH3
MYP_2588
xylosidase/arabinosidase
xylosidase/arabinosidase
GH3
MYP_3067
glycosidehydrolase
GH3
MYP_4245
xylosidase/arabinosidase
xylosidase/arabinosidase
GH3
MYP_4961
b-N-acetylglucosaminidase
GH5
MYP_0458
endoglucanase
Endoglucanase
GH5
MYP_2390
endoglucanase
endoglucanase
GH5
MYP_1753
beta-glycosidase
beta-glycosidase
GH5
MYP_2157
endo-1,4-beta-mannosidase
endo-1,4-beta-mannosidase
GH5
MYP_2462
beta-glycosidase
beta-glycosidase
GH5
MYP_2666
endoglucanase
endoglucanase
GH5
MYP_3206
endo-1,4-beta-mannosidase
endo-1,4-beta-mannosidase
GH5
MYP_3455
putativecarbohydrate-activeenzyme
GH5
MYP_3567
endoglucanase
endoglucanase
GH8
MYP_0051
beta-glycosidase-likeprotein
beta-glycosidase-likeprotein
GH8
MYP_1583
b-glycosidase
b-glycosidase
GH8
MYP_1794
chitinase
Chitinase
GH8
MYP_2076
hypotheticalprotein
GH8
MYP_2112
bifunctionalxylanase/esterase
bifunctionalxylanase/esterase
GH8
MYP_2156
beta-glycosidase-likeprotein
beta-glycosidase-likeprotein
GH8
MYP_2756
chitosanase-glucanase
chitosanase-glucanase
GH8
MYP_3325
beta-glycosidase
beta-glycosidase-likeprotein
GH8
MYP_3614
bifunctionalxylanase/esterase
bifunctionalxylanase/esterase
GH8
MYP_3786
hypotheticalprotein
GH8
MYP_4347
CBM4_9
glycosidehydrolase
GH8
MYP_4354
glycosidehydrolase
GH9
MYP_0135
Endoglucanase-likeprotein
GH9
MYP_0327
endoglucanase
Endoglucanase
GH9
MYP_1185
endoglucanase
GH9
MYP_1436
hypotheticalprotein
GH9
MYP_1796
endoglucanase
endoglucanase
GH9
MYP_2099
hypotheticalprotein
GH9
MYP_3429
beta-glycosidase
beta-glycosidase-likeprotein
GH9
MYP_4968
endoglucanase
endoglucanase
GH10
MYP_2074
CBM4_9
bifunctionalacetylxylanesterase/xylanase
GH10
MYP_2104
CBM6
endoglucanase-likeprotein
endoglucanase-likeprotein
GH10
MYP_3331
CBM6
xylanase
Xylanase
GH10
MYP_4086
bifunctionalacetylxylanesterase/xylanase
GH11
MYP_2111
CBM9
xylanase
Xylanase
GH13
MYP_2299
alpha-amylase
alpha-amylase
GH15
MYP_0927
hypotheticalprotein
GH15
MYP_3689
glucoamylase
glucoamylase
GH16
MYP_3454
CBM4
glycosidehydrolasefamilyprotein
GH16
MYP_4151
glycosidehydrolasefamily16
GH16
MYP_4349
CBM4_9
Beta-glucanase
Beta-glucanase
GH18
MYP_3548
glycosidehydrolase
GH26
MYP_4610
hypotheticalprotein
GH30
MYP_2082
CBM6
carbohydrate-bindingfamily6protein
glucuronoxylanase
GH31
MYP_0331
a-glucosidase
GH39
MYP_0836
CBM6
hypotheticalprotein
GH43
MYP_2103
CBM6
beta-xylosidase/
alpha-L-arabinofuranosidase
beta-xylosidase/
alpha-L-arabinofuranosidase-likeprotein
GH43
MYP_2107
CBM6
hypotheticalprotein
GH43
MYP_2166
CBM6
multifunctionalacetylxylanesterase/b-xylosidase/a-L-arabinofuranosidase
GH43
MYP_3646
Arabinose
GH53
MYP_3037
hypotheticalprotein
GH57
MYP_0329
alpha-amylase
alpha-amylase
GH63
MYP_0745
hypotheticalprotein
GH63
MYP_2463
hypotheticalprotein
GH65
MYP_2960
trehalose6-phosphatephosphorylase
GH97
MYP_3104
alpha-glucosidase
alpha-glucosidase
GH108
MYP_0649
hypotheticalprotein
PL
（2）
PL3
MYP_4611
pectatelyase
pectatelyase
PL3
MYP_4344
pectatelyase
CE（10）
CE
MYP_1590
esterase/lipase
CE
MYP_2110
esterase
CE
MYP_2444
polysaccharidedeacetylase
CE
MYP_3048
arylesterase
CE
MYP_3055
acetylxylanesterase
CE
MYP_3791
UDP-3-O-N-acetylglucosaminedeacetylase
CE
MYP_3868
Pectinesterase
CE
MYP_4162
carboxylesterase
CE
MYP_4267
polysaccharidedeacetylase
CE
MYP_4670
esterase
GT（90）
GT1
MYP_0230
3-deoxy-D-manno-octulosonicacidtransferase
GT1
MYP_0330
hypotheticalprotein
GT1
MYP_0486
hypotheticalprotein
GT1
MYP_0985
Dolichol-phosphatemannosyltransferase
GT1
MYP_1016
hypotheticalprotein
GT1
MYP_1017
hypotheticalprotein
GT1
MYP_1020
hypotheticalprotein
GT1
MYP_1284
glycosyltransferase
GT1
MYP_1285
hypotheticalprotein
GT1
MYP_1289
a-glycosyltransferase
GT1
MYP_1290
a-glycosyltransferase
GT1
MYP_1293
a-glycosyltransferase
GT1
MYP_1644
a-glycosyltransferase
GT1
MYP_2180
a-glycosyltransferase
GT1
MYP_2422
group1glycosyltransferase
GT1
MYP_2494
glycosyltransferas
GT1
MYP_3185
hypotheticalprotein
GT1
MYP_3186
glycosyltransferase
GT1
MYP_3279
glycosyltransferase
GT1
MYP_3393
SAM-dependentmethyltransferase
GT1
MYP_3652
hypotheticalprotein
GT1
MYP_3933
hypotheticalprotein
GT1
MYP_4319
hypotheticalprotein
GT1
MYP_0560
hypotheticalprotein
GT1
MYP_2014
hypotheticalprotein
GT1
MYP_2360
a-glycosyltransferase
GT1
MYP_2361
hypotheticalprotein
GT1
MYP_2530
hypotheticalprotein
GT1
MYP_3742
glycosyltransferas
GT1
MYP_4038
hypotheticalprotein
GT1
MYP_4234
glycosyltransferase
GT1/GT2
MYP_1046
hypotheticalprotein
GT2
MYP_0044
hypotheticalprotein
GT2
MYP_0228
family2glycosyltransferase
GT2
MYP_0507
glycosyltransferasefamily2
GT2
MYP_0557
putativetransmembraneglycosyltransferase
GT2
MYP_0564
glycosyltransferasefamilyprotein
GT2
MYP_0573
glycosyltransferasefamily2
GT2
MYP_1011
glycosyltransferase
GT2
MYP_1012
hypotheticalprotein
GT2
MYP_1013
family2glycosyltransferase
GT2
MYP_1014
glycosyltransferasefamily2
GT2
MYP_1047
glycosyltransferase
GT2
MYP_1048
hypotheticalprotein
GT2
MYP_1249
hypotheticalprotein
GT2
MYP_1276
glycosyltransferasefamily2
GT2
MYP_1281
family2glycosyltransferase
GT2
MYP_1291
putativecolanicacidbiosynthesisacetyltransferase
GT2
MYP_1316
hypotheticalprotein
GT2
MYP_1673
glycosyltransferase
GT2
MYP_1684
a-glycosyltransferase
GT2
MYP_2040
glycosyltransferasefamily2
GT2
MYP_2178
family2glycosyltransferase
GT2
MYP_2187
hypotheticalprotein
GT2
MYP_2239
family2glycosyltransferase
GT2
MYP_2483
glycosyltransferasefamily2
GT2
MYP_2489
glycosyltransferasefamily2
GT2
MYP_2490
glycosyltransferasefamily2
GT2
MYP_3094
hypotheticalprotein
GT2
MYP_3177
hypotheticalprotein
GT2
MYP_3228
glycosyltransferasefamily2
GT2
MYP_3390
glycosyltransferasefamily2
GT2
MYP_3391
b-glycosyltransferase
GT2
MYP_3497
dolichyl-phosphatebeta-D-mannosyltransferase
GT2
MYP_3817
dolichol-phosphatemannosyltransferase
GT2
MYP_3927
glycosyltransferasefamily2
GT2
MYP_3935
hypotheticalprotein
GT2
MYP_3936
glycosyltransferase
GT2
MYP_3938
hypotheticalprotein
GT2
MYP_3941
putativeacetyltransferase
GT2
MYP_4271
glycosyltransferasefamily2
GT2
MYP_4449
b-glycosyltransferase
GT2
MYP_4494
family2glycosyltransferase
GT3
MYP_1785
anthranilatephosphoribosyltransferase
GT3
MYP_2499
hypotheticalprotein
GT4
MYP_0931
glycosyltransferase/acetylmuramylpentapeptidephosphotransferase
GT4
MYP_4218
phospho-N-acetylmuramoyl-pentapeptide-transferase
GT5
MYP_2032
glycogensynthase
GT9
MYP_3183
hypotheticalprotein
GT9
MYP_3184
glycosyltransferasefamily9
GT9
MYP_3190
glycosyltransferase
GT9
MYP_1186
glycosyltransferase
GT9
MYP_1410
heptosyltransferase
GT9
MYP_2362
hypotheticalprotein
GT9
MYP_4154
glycosyltransferasefamily9
GT20
MYP_2116
hypotheticalprotein
GT20
MYP_3131
hypotheticalprotein
GT20
MYP_3690
hypotheticalprotein
GT25
MYP_0631
glycosyltransferase
GT28
MYP_4221
UDP-diphospho-muramoylpentapeptidebeta-N-acetylglucosaminyltransferase
CBM（8）
CBM6
MYP_1233
CBM6
carbohydratebindingmodule
CBM6
MYP_2105
CBM6
carbohydratebindingmodule
CBM6
MYP_2109
CBM6
carbohydratebindingmodule
CBM6
MYP_2162
CBM6
carbohydratebindingmodule
CBM6
MYP_2165
CBM6
carbohydratebindingmodule
CBM4
MYP_3691
CBM4
carbohydratebindingmodule
CBM6
MYP_4094
CBM6
carbohydratebindingmodule
CBM6
MYP_4095
CBM6
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