华南理工大学动物实验申请.docx
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华南理工大学动物实验申请
华南理工大学动物实验申请
SouthChinaUniversityofTechnologyAnimalProtocol
动物伦理号:
AnimalEthicsCommitteeNumber
提交日期:
DateSubmitted
批准日期:
ApprovalDate
PROPOSAL#:
APPROVALDATE:
EXPIRATIONDATE:
PPOSAL#:
ROPOSAL#:
PROPOSAL#:
APPROVALDATE:
EXPIRATIONDATE:
A1.项目资料ApplicationData
项目名称ProjectTitle
首次提交Initialsubmission:
[]
更新Renewal:
[]
修改Modification:
[]
项目来源FundingSource
B1.课题组负责人(PI)PrincipalInvestigator(Co-Investigatorsmayfollow)
姓名Name
单位Unit
部门Department
手机号码MP
办公电话TEL
邮箱E-mai
B2.课题组负责人是否亲自操作?
DoesthePIhavedirectcontactwithanimals?
[]是Yes
[]否No
B3.动物实验操作人员Whowillperformtheexperimentalmanipulationsonanimals?
姓名
Name
曾开展动物实验的种类和期限YearsofWorkingExperiencewiththeSpecies
何时何地获得的工作经验
When&WhereExperienceReceived
手机号码
MobilPhone
邮箱
E-mail
可增加行Repeatrowswhenneeded
B4.操作者是否有相关的操作经验?
Arethepersonsperformingtheexperimentsorsurgerieswithanimalsexperiencedwiththespecificproceduresinvolved?
[]是Yes
[]否。
注明他们在进行实验之前如何受到相关培训
No.Indicatehowtheywillreceivetrainingbeforetheexperimentsbegin
[]由PI培训TrainingbyPI
[]由实验动物中心培训Trainingbylaboratoryanimalcenter
[]由其它单位或个人培训(姓名)Trainingbyother(Name)
C1.实验的目的或预期目标是什么?
Whataretheobjectivesorunderlyinghypothesesoftheexperiments?
C2.你的实验最终如何让患者(人或动物)受益?
Howmightapatientpopulation(humanoranimal)ultimatelybenefitfromyourexperiments?
C3.动物使用原理(叙述使用动物的理由,证明所选物种的适宜性)
Explainyourrationaleforanimaluse.[Therationaleshouldincludereasonswhyitisnecessarytouseanimalmodels.]Justifytheappropriatenessofthespeciesselected.[Thespeciesselectedshouldbethelowestpossibleonthephylogeneticscale.]
C4.请详述所采用的动物实验程序(涉及动物的实验方法及操作顺序,药物的剂量、给药方式、药物剂型、取样方法和次数等)
Provideacompletedescriptionoftheprocedure(s)tobeperformedusinganimals(i.e.,natureandtimesequenceofanimalrelatedprocedures,dose/route/regimenofdrugadministration,samplingmethodsandfrequencies,etc.)
C5.所需动物数量。
Numberofanimalsrequested(byspecies).
种
Species
品种/品系
Strain/Stock
性别
Sex
年龄
Age
重量
Weight
突变系的数量NumberofMutantStrains
动物总数
TotalNumberofAnimals
可增加行Repeatrowswhenneeded
C6.请说明所需实验动物数量的科学依据。
列出每次动物实验所需的动物数量和每个实验所用到的对照组动物数目。
Providescientificjustificationforthenumberofanimalsrequested.Listthenumberofanimalsforeachexperimentalandcontrolgroupforeachexperiment.
C7.是否需要特殊的护理条件(如笼具、温度、光照、卫生条件、食物、饮水等)。
Willspecialhusbandryconditionsbeneeded(e.g.,caging,temperature,lightcycle,sanitation,diet,water,etc.)?
[]是Yes
[]否No
[]若采血,请填写下表IfsectionDisapplicable,Completethetablebelow.
[]不采血SectionDisNOTapplicable
品种/品系
Species/Strain
采血量
VolumetoBeCollected
采血周期
FrequencyofCollection
采血方法
MethodofCollection
麻醉或镇静AnesthesiaorSedationUsed
可增加行Repeatrowswhenneeded
[]若适用,请填写E1-E4项。
IfsectionEisapplicable,completeE1-E4.
[]不适用。
SectionEisNOTapplicable.
E1.品种/品系Species/Strain
E2.何时镇静Whenissedationused?
E3.药名Drugname剂量Dose给药方式Route
E4.Howwillthedepthofanesthesiabemonitored?
如何监控麻醉深度
[]脚趾收缩反应丧失Absenceofwithdrawalreflextotoepinc
[]眼脸反应消失Absenceofblinkreflex
[]其它Other
[]如做手术,请完成F1-F13。
IfsectionFisapplicable,completeF1-F13.
[]不做手术。
SectionFisNOTapplicable.
术前准备事项PRE-OPERATIVECARE
F1.实验动物需要术前用药吗?
Willtheanimalrequirepre-operativedrugs?
[]需要Yes
药名Drugname剂量Dose途径Route周期Frequency
[]不需要No
Withholdingfoodandwaterisnotrecommendedforrodents.不建议对啮齿动物采取禁食禁水。
F2.是否让实验动物存活?
Isthisasurvivalprocedure?
[]是Yes
[]否No
Theanimalwillbeeuthanizedafteranesthesia.请将动物麻醉后安乐处死.
F3.是否是大手术?
Isthisa“major”procedure?
[]是Yes
[]否No
F4.是否为多重存活手术?
Willmorethanonesurvivalprocedurebeperformedonanyoneanimal?
[]如是,请提供科学依据。
Yes.Ifso,providescientificjustification.
[]没有No.
F5.手术过程的描述Describethesurgicalprocedure(s)
F6.怎样缝合皮肤?
Howwillskinbeclosed?
[]夹子或不可吸收性缝合Clipsornon-absorbablesuture
Mustberemovednolaterthantwoweekspost-op.夹子或不可吸收性缝合须在术后两周内去除。
[]其它方法(请描述)Other(Pleasedescribe)
F7.手术操作者?
Whowillperformthesurgicalprocedure?
F8.手术将在一般消毒条件还是无菌条件下进行?
Willsurgerytakeplaceundersterileconditionsorasepticconditions?
[]消毒Sterile
[]无菌Aseptic
啮齿类动物手术可以在无菌操作条件下进行,要求
(1)手术区域干净[用消毒液擦拭过(例如碘酊和75%乙醇溶液)],
(2)器械经高压灭菌或化学消毒15min以上,手术相关人员要戴口罩、无菌手套或用75%乙醇或其他消毒剂彻底清洗消毒过的乳胶手套。
Rodentsurgerycanbeperformedunderasepticconditions,meaningthat:
(1)thesurgicalareaisclean[wipeddownwithadisinfectant(i.e.,75%alcoholandIodinesolution)],
(2)instrumentsaresterilizedeitherbyautoclavinginstrumentpackorbysoakinginachemicalsterilant(Instracal,Amerse)foratleast15minutes,and(3)thesurgeonwearsamaskandsterileglovesorlatexglovesthoroughlycleanedwith75%ethanol,oranothergermicidalagent.
术后护理POST-OPERATIVECARE
要求在动物恢复期间对其定时进行观察和翻身。
Itisrequiredthatanimalsmustbeobservedduringrecoveryandmustbeturnedfromsidetosideperiodically.
F9.术后护理人员?
Whowillperformpost-operativecare?
F10.手术过程中和术后需要采取哪些预防措施防止动物体温下降或脱水?
Whatprecautionswillbetakenduringandaftersurgerytopreventhypothermiaand/ordehydration?
[]加热灯Heatinglamp[]静脉注射Intravenousfluids
[]加热垫Warmingblanket[]皮下注射Subcutaneousfluids
[]腹腔注射Intraperitonealfluid
F11.手术动物返回饲养笼前的判断标准Whatcriteriawillbeusedtodeterminethatanimalsarereadytoreturntotheirhousing?
[]动物可以腹卧Sternalrecumbency
需持续观察到动物能够腹卧。
Observedcontinuouslyuntiltheycanlieontheirstomachs.
[]其它Other:
F12.采用何种药物术后止痛Whatpost-operativeanalgesicswillbeused?
如果手术后会带来疼痛应该对动物进行镇痛。
那些能够在人身上对于术后镇痛有作用的药物对动物也同样适用。
Theanimalshouldbegivenananalgesicifthereisapossibilityofpost-operativepain.Ifahumanwouldbegivenpainmedicationsafterasimilarprocedure,thenanalgesicsshouldbeprovidedfortheanimal.
[]没有None
[]药名Name剂量Dosage途径Route周期Frequency
需用给予止痛药的判定体征Indicationsforanalgesics
[]活动受限Restrictedmobility[]食欲下降Poorfoodintake
[]其它Other:
[]止痛药将会按惯例使用而不是需要时才使用。
Analgesicswillbegivenroutinely,asopposedtoan“as-needed”basis.
F13.手术后用何种抗生素?
Whatpost-operativeantibioticswillbeused?
[]不用None
[]名称Name剂量Dosage途径Route周期Frequency
需用抗生素的指征Indicationsforantibiotics:
[]预防Prophylaxis[]特别症状Specificindications
每项申请都需填报此栏ThisSectionmustbecompletedforeveryprotocol.
G1.动物在有何种症状时采用安乐死?
Whatcriteriawillbeusedforearlyeuthanasia?
每一项实验都难以避免有一些并发症的产生,所以动物需及早进行安乐死,每个PI应提供针对性的、客观的标准来确定对动物实施安乐死时机。
Everyexperimentcarriesthepossibility,howeverunlikely,ofunforeseencomplications,whichmightrequireearlyeuthanasiaofananimal.Everyinvestigatorshouldindicatespecific,objectivecriteriathatwillbeusedtoidentifyanimalsthatshouldbeeuthanized.
如果动物开始表现有不动、蜷缩、拒食、皮毛皱褶、自虐等征兆,立即对动物实施安乐死。
Iftheanimalstartsshowingsignsofimmobility,ahuddledposture,theinabilitytoeat,ruffledfurorself-mutilation,theanimalwillbesacrificedimmediately.
G2.是否有无法排减的疼痛和痛苦(例如疼痛持续时间长或剧痛)?
Willtherebeunrelievedpainorstress(definedaslastingformorethanamoment,i.e.,longerdurationormorepainfulthananeedlestick)?
[]有Yes
[]没有No
如果有,提供科学依据。
止痛药可以减少疼痛,但是却不能减轻动物的心理压力。
因此应当有给动物减压的措施,比如让动物提前适应环境等,将改善措施做到极致。
Ifyes,youmustprovidescientificjustification.Paincanbealleviatedwithanalgesics,butstressisnotalleviatedbypainmedications.Itshouldbeclearthatadjunctivemeasurestominimizestress,suchasreassuranceandpriorhabitationtothestressfulenvironment,havebeenimplementedtothefullestextent.
G3.实验动物是否允许人道终点?
Willprotocolinvolvedeathasanendpoint?
[]允许Yes
[]否No
如是则每天应由专人对病危的动物进行观察记录两次(早晚各一次)。
当发现动物有死亡征兆时,如出现蜷缩、一动不动、皮毛皱褶、不进食、体温降低(直肠温度﹤34℃)或体重下降(﹥20%)等症状时,经慎重考虑后对动物实施安乐死。
如动物不能站立,呼吸困难,严重的肌肉萎缩,严重溃疡或流血不止时应及时采用适当的方法让其安乐死。
Ifthisalternativeisconsidered,youmustaddressthefollowingissuesinthespacebelow.Itisconsideredtobemorehumanetoeuthanizeanimalswhenitcanbereasonablypredictedthattheanimalwilldiesoon.Indicatorssuchashuddledposture,immobility,ruffledfur,failuretoeat,hypothermia(colonictemperatureof<34oC),orweightloss/(>20%)maybeusefulobjectivecriteriaforearlyeuthanasia.Animalsapproachingapre-moribundstateshouldbemonitoredtwiceaday(atthebeginningandendoftheday)byanexperiencedperson.Writtenrecordsofthemonitoringsessionshouldbemaintained.Animalsshoulddefinitelybeeuthanizediftheyareunabletostandoriftheydisplayagonalbreathing,severemuscularatrophy,severeulceration,oruncontrolledbleeding.
H1.方法Methods
[]麻醉过量Drugoverdose
[]三倍剂量的复合麻醉剂
Tripletherecommendedanesthesiadoseforthecombinationanesthesia.
[]戊巴比妥钠120mg/kg,大小鼠静脉注射
Pentobarbitalsodium,120mg/kgIP,IVinrodents.
[]CO2吸入CO2inhalationc.
Approvedforrodents.适用于啮齿动物。
[]麻醉后颈椎脱臼Cervicaldislocationunderanesthesia
Approvedformiceandratslessthan200grams.适用于小鼠和体重小于200克的大鼠。
[]麻醉后断颈Decapitationunderanesthesia
[]麻醉后放血Exsanguinationunderanesthesia
[]其它(请说明)Other(Describe.)
I1.实验过程中是否使用有毒有害物质,包括放射性物质、病原微生物、危险化学药品等?
Willproceduresutilizepotentiallyhazardousmaterials,includingradioactivesubstances,infectiousmicroorganism,hazardouschemicals,andhazardouswaste?
[]有,请列出Yes.Pleaselist.
[]没有No
I2.相关物品的安全资料是否方便索取?
Arematerialsafetydatasheets(MSDSs)readilyaccessibletoaffectedpersonnelforallchemicalsthatwillbeused(e.g.,an