蛋白质相关实验技术操作指南Word文档下载推荐.doc

蛋白质相关实验技术操作指南Word文档下载推荐.doc

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ProbingandStrippingofWesternBlot

HahnLab，TheFredHutchinsonCancerResearchCenterandHowardHughesMedicalInstitute

WesternBlottingProtocol

MirmiraLaboratoryattheUniversityofVirginia

WesternBlottingandImmunostaining

ChazinLab,CenterforStructuralBiology,VanderbiltUniversity

WesternBlots

LabofDr.MarkBartonFrank,OklahomaMedicalResearchFoundation

WesternBlotting-Antibodies

TheMinionLab,CollegeofVeterinaryMedicineatIowaStateUniversity

WesternBlotting

Cepko/TabinLab,HarvardUniversity

CarbonateSolutionForWesternBlot

Dr.DEKoshland,CarnegieInstitutionofWashington

WesternBlotwithBSA

METHODforWesternBlots

MichaelKoelle'

sLab,DepartmentofMolecularBiophysicsandBiochemistry

YaleUniversity

2、蛋白分离/提取/浓缩

LysisBuffers

YeastProteinIsolation"

Yaffe-Schatz"

AmbergLab,UpstateMedicalUniversity

GuanidineHydrochloridePurificationofProteinsFromSDSPAGE

GottschlingLab，TheFredHutchinsonCancerResearchCenterandHowardHughesMedicalInstitute

YeastproteinprepforSDSPAGEandwestern（rapid）

YeastproteinprepforSDSPAGEandwestern（glassbead）

PurificationofTFIIICfromyeast

PurificationofTFIIAfromE.coli

TCAproteinprecipitation

PurificationofMLCK（MyosinLightChainKinase）fromRabbitSkeletalMuscle肌球蛋白轻链激酶

StullLab,UniversityofTexas,SouthwesternMedicalCenter

PurificationofCalmodulinfromBovineBrain

StullLab,UniversityofTexas,SouthwesternMedicalCenter

MyosinLightChainPreparationfromSkeletalandCardiacMuscle

PurifyingProteinfromInclusionBodies

ChazinLab,CenterforStructuralBiology,VanderbiltUniversity

ProtocolforProteinExtractionfromPlant

TheUniversityofNebraska-LincolnProteinCoreFacility（PCF）

TaqPolymerasePurification

YeastProteinPrep（NoBoil）

YeastProteinPrep（Boil）

GSTFusionProteinPrep

VesicleTrafficking,StanfordUniversity

3、蛋白质检测分析：

AMIDOBLACKSTAIN

HancockLaboratoryMethods,DepartmentofMicrobiologyandImmunology,

UniversityofBritishColumbia,BritishColumbia,Canada

JamesHardwick'

sangiotensinassayprotocol

SeftonLab,SalkInstitute

Phosphoaminoacidanalysis:

MarkKamps'

smethod

SeftonLab,SalkInstitute

ULTIMATEHIS-UBASSAYFOR

theTanseyLabatColdSpringHarborLaboratory

ULTIMATEHIS-UBASSAYFORYEAST

ProtocolforBeta-HexosaminidaseRBL-2H3SecretionAssay

meyerlab,StanfordUniversity

MeasurementofMyosinRegulatoryLightChain（RLC）Phosphorylation

BicinchoninicAcid（BCA）ProteinAssay

DavidR.Caprette,RiceUniversity

BiuretProteinAssay

Bradfordproteinassay

Hartree-LowryandModifiedLowryProteinAssays

ColorimetricAssays

Kdmeasurement,fitting,calculation,andsimulation

4、蛋白标记修饰

DSPCrosslinking

PeterNovickLab,DepartmentofCellBiologyYaleUniversitySchoolofMedicine

LabelingGc-GlobulinwithCarboxyfluoresceinSuccinimidylEster

Yu-LiWangLab,UniversityofmassachusettsMedicalSchool（UMASS）

LabelingTubulinwithTetramethylrhodamineSuccinimidylEster

LabelingGizzardVinculinwithTetramethylrhodamineIodoacetamide

LabelingGizzardAlpha-actininwithTetramethylrhodamineIodoacetamide

LabelingGizzardMyosinwithTetramethylrhodamineIodoacetamide（primarily17kDMLC）

LabelingGizzardMyosinwithTetramethyorhodamineIodoacetamide（MHCand17kDMLC）

LabelingMuscleActinwithN-（1-pyrene）Iodoacetamide

LabelingMuscleActinwithTetramethylrhodamineIodoacetamide

Yu-LiWangLab,UniversityofmassachusettsMedicalSchool（UMASS）

LabelingMuscleActinwithCarboxyfluoresceinSuccinimidyleEster

LabelingMuscleActinwith5-Iodoacetamidofluorescein

ultimatemammaliancellpulse-chase

ultimateyeastpulse-chase

GluteraldehydeConjugationofOligopeptidestoProteins

Fluoresceinlabelingofproteins

DavidChambers,Salkinstitute

Biosyntheticlabeling

SeftonLab,SalkInstitute,SanDiego,California

5、免疫沉淀（IP/CoIP/ChIP）

Immunoprecipitationbuffersandprotocols

CellLysis/Western/IP

AmplificationandLabellingwithCydyes

YaleGenomeAnalysisCenter,YaleUniversity

ChromatinImmunoprecipitationfromMammalianCellExtracts

ChromatinImmunoprecipitationfromYeastWholeCellExtracts

Immunoprecipitation

HancockLaboratoryMethods.DepartmentofMicrobiologyandImmunology,

UltimateyeastdenaturingIP

ULTIMATEFREEZE-THAWLYSISFOR

UltimatemammaliannondenaturingIP

UltimatemammaliandenaturingIP

theTanseyLabatColdSpringHarborLaboratory

UltimateyeastChIPassay

theTanseyLabatColdSpringHarborLaboratory

ChromatinImmunoprecipitationAssayandPCR

YeastChromatinImmunoprecipitation（ChIP）

MirmiraLabChIPProtocol

ChIPAssayProtocol

ImmunoprecipitationandImmuneComplexkinaseassay

GeneralPrinciplesofImmunoprecipitation

6、融合蛋白表达及纯化

UseofB-PERlysisreagenttopurifyrecombinantproteinstaggedwithHis6ormaltosebindingprotein

SmithLab,DivisionofBiologicalSciences,UniversityofMissouri

BacterialExpressionofKinesinMotors

LizGreeneandSteveHenikoff

PurificationofrecombinantsBRFM166L

ProteinExpressionandPurificationofRPA70-AB

Co-ExpressionandpurificationofhumanDNAprimasep49-p58subunits引发酶p49-p58亚基共表达纯化方法

ProteinExpressionandPurificationofMBP-TantigenMBP-T抗原表达纯化

PerspectivesonBaculovirusExpressionSystems表达

Expression&

PurificationofHisTaggedProteinsinE.coli

7、相互作用分析

Amplifyingalargephage-displaylibrarywithout