各种缓冲溶液配制表-.docx

各种缓冲溶液配制表-.docx

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各种缓冲溶液配制表-

实验室常用缓冲液的配制方法

1.甘氨酸-盐酸缓冲液（0.05M）

Xml0.2M甘氨酸+Yml0.2M盐酸，再加水稀释至200ml。

pHX/mlY/mlpHX/mlY/ml

2.25044.03.05011.4

2.45032.43.2508.2

2.65024.23.4506.4

2.85016.83.6505.0

甘氨酸分子量=75.070.2M甘氨酸溶液含15.01g/L

2.邻苯二甲酸-盐酸缓冲液（0.05M）

Xml0.2M邻苯二甲酸氢钾+Yml0.2M盐酸，再加水稀释至200ml。

pHX/mlY/mlpHX/mlY/ml

2.254.6703.251.470

2.453.9603.450.990

2.653.2953.650.597

2.852.6423.850.263

3.052.032

邻苯二甲酸氢钾分子量=204.230.2M邻苯二甲酸氢钾溶液含40.85g/L

3.磷酸氢二钠-柠檬酸缓冲液

pH0.2MNa2HPO4/ml0.1M柠檬酸/mlpH0.2MNa2HPO4/ml0.1M柠檬酸/ml

2.20.4019.65.210.729.28

2.41.2418.765.411.158.85

2.62.1817.825.611.608.40

2.83.1716.835.812.097.91

3.04.1115.896.012.637.37

3.24.9415.066.213.226.78

3.45.7014.306.413.856.15

3.66.4413.566.614.555.45

3.87.1012.906.815.454.55

4.07.7112.297.016.473.53

4.28.2811.727.217.392.61

4.48.8211.187.418.171.83

4.69.3510.657.618.731.27

4.89.8610.147.819.150.85

5.010.309.708.019.450.55

Na2HPO4分子量=41.980.2M溶液含28.40g/L

Na2HPO4·2H2O分子量=78.050.2M溶液含35.61g

C6H8O7·H2O分子量=10.140.1M溶液含21.01g/L

4.柠檬酸-氢氧化钠-盐酸缓冲液

pH钠离子浓度/M柠檬酸C6H8O7·H2O/g氢氧化钠NaOH/g浓盐酸HCl/ml终体积/L

2.20.202108416010

3.10.202108311610

3.30.202108310610

4.30.20210834510

5.30.352451446810

5.80.4528518610510

6.50.3826615612610

使用时可以每升中加入1g酚，若最后pH有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。

柠檬酸-柠檬酸钠缓冲液（0.1M）

pH0.1M柠檬酸/ml0.1M柠檬酸钠/mlpH0.1M柠檬酸/ml0.1M柠檬酸钠/ml

3.018.61.45.08.211.8

3.217.22.85.27.312.7

3.416.04.05.46.413.6

3.614.95.15.65.514.5

3.814.06.05.84.715.3

4.013.16.96.03.816.2

4.212.37.76.22.817.2

4.411.48.66.42.018

4.610.39.76.61.418.6

4.89.210.8

C6H8O7·H2O分子量=10.140.1M溶液含21.01g/L

Na3C6H5O7·2H2O分子量=294.120.1M溶液含29.41g/L

5.乙酸-乙酸钠缓冲液（0.2M）

pH0.2MNaAc/ml0.2MHAC/mlpH0.2MNaAc/ml0.2MHAC/ml

3.60.759.254.85.904.10

3.81.208.805.07.003.00

4.01.808.205.27.902.10

4.22.657.355.48.601.40

4.43.706.305.69.100.90

4.64.905.105.89.400.60

NaAc分子量=136.090.2M溶液为27.22g/L

6.磷酸盐缓冲液

（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2M）详见常用磷酸缓冲液的配制方法

（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15M=cM）

pHcMNaH2PO4/mlcMKH2PO4/mlpHcMNaH2PO4/mlcMKH2PO4/ml

4.920.109.907.177.003.00

5.290.509.507.388.002.00

5.911.009.007.739.001.00

6.242.008.008.049.500.50

6.473.007.008.349.750.25

6.644.006.008.679.900.10

6.815.005.009.1810.000

6.986.004.00

Na2HPO4·2H2O分子量=178.05cM溶液含35.61g/L

KH2PO4分子量=136.09cM溶液含9.078g/L

（3）磷酸二氢钾-氢氧化钠缓冲液（0.05M）

Xml0.2MKH2PO4+Yml0.2MNaOH，再加水稀释至20ml。

pH（20℃）X/mlY/mlpH（20℃）X/mlY/ml

5.850.3727.052.963

6.050.5707.253.500

6.250.8607.453.950

6.451.2607.654.280

6.651.7807.854.520

6.852.3658.054.680

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