基因.docx

1、基因生物学基因部分核酸 nucleic acid核苷酸 nucleotide脱氧核糖核酸deoxyribonucleic acid(DNA)核糖核酸ribonucleic acid(RNA)寡核苷酸 oligonucleotide序列 sequence修饰 modify/modification重组 recombination/recombinant载体 vector宿主 host本发明提供了含有编码修饰细胞色素P450酶的核苷酸序列的核酸；以及含有该核酸的重组载体和宿主细胞。The present invention provides nucleic acids comprising nuc

2、leotide sequences encoding modified cytochrome P450 enzymes; as well as recombinant vectors and host cells comprising the nucleic acids.构建 construct表达 expression本发明公开了制备所述酚氧化酶的方法以及构建表达宿主的方法。Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing exp

3、ression hosts.调节 regulate/regulation启动子 promoter终止子 terminator本发明还涉及编码这些蛋白质的核酸序列，特别是调节相应基因表达的启动子序列。The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes.多核苷酸 polynucleotide鉴定 identifi

4、cation分离 isolation本发明涉及一种编码由致病杆菌属（Xenorhabdus）和光杆状菌属（Photorhabdus）细菌产生的对昆虫具有特异性的新型蛋白毒素的多核苷酸分子鉴定和分离。The invention relates to the identification and isolation of polynucleotide molecules encoding a new class of protein insecticidal toxins which are produced by bacteria from the genera Xenorhabdus and

5、Photorhabdus. 族 family前体 precursor编码 code for本发明提供了一族新的肿瘤排斥抗原前体，编码它们的核酸分子。这些肿瘤排斥抗原前体被称作肿瘤排斥抗原前体，编码它们的核酸分子被称作编码分子。本发明还提供了所说编码序列、肿瘤排斥抗原和其前体分子的各种诊断和治疗用途。A new family of tumor rejection antigen precursors, and the nucleic acid molecules which code for them, are disclosed. These tumor rejection antigen p

6、recursors are referred to as DAGE tumor rejection antigen precursors, and the nucleic acid molecules which code for them are referred to as GAGE coding molecules. Various diagnostic and therapeutic uses of the coding sequences and the tumor rejection antigens, and their precursor molecules are descr

7、ibed.底物特异性 substrate specificity突变体mutant 诱变mutagenesis本发明提供了显示出改变的底物特异性的新的突变内酰胺青霉素酰基转移酶。New mutant beta -lactam Penicillin G acylases are provided, exhibiting altered substrate specificities.本发明涉及用于引导突变进入分子的诱变方法，特别地涉及可以运用于分子群以产生或筛选涉及多个分子突变的文库的方法。The present invention relates to a method of mutagene

8、sis for introducing mutations into a molecule and, in particular, to methods that may be applied to populations of molecules for the generation or screening of libraries involving the mutation of multiple molecules.筛选 screenAT含量 AT contentGC含量 GC content表型 phenotype基因型 genotype缺陷 deficient生物合成 biosy

9、nthesis筛选在疟原虫中抑制GPI生物合成的化合物的方法 此外，本发明人揭示比编码PfGWT1蛋白的DNA 具有更低AT含量的简并突变体DNA，能补偿GWT1-缺陷型酵母的表型。Methods of screening for compounds that inhibit the biosynthesis of GPI in malaria parasitesIn addition, the inventors revealed that degenerate mutant DNAs, with a lower AT content than the DNA encoding the Pf

10、GWT1 protein, can complement the phenotype of GWT1-deficient yeast. 体外 in vitro体内 in vivo克隆 clone区段 segment位点 site嵌合 chimeric基因工程gene engineering本发明提供了用核酸、载体及体外和体内方法进行的重组克隆方法，可用来经改造过的重组位点和重组蛋白质移动或交换DNA分子区段，得到具有预期特性的嵌合DNA分子和/或DNA区段。Recombinational cloning is provided by the use of nucleic acids, vect

11、ors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).转染transfect/tansfection标记 marker所述遗传改变的细胞已用遗传物质转染，

12、从而以组成型或受控的方式表达标记基因和至少一种治疗基因。The genetically-altered cells are transfected with genetic material for expressing a marker gene and at least one therapeutic gene in a constitutively or controlled manner. mRNA干扰 mRNA interference, RNAimRNA干扰酶 mRNA interferase切割cleave/cleavage上游upstream 下游 downstream调节剂/

13、调节物regulator凋亡 apoptosis促凋亡的 proapoptotic诱导 induce沉默silence本发明提供组合物和方法，所述组合物和方法通过将包含siRNA分子的核酸-脂质颗粒传递给细胞使基因表达沉默。The present invention provides compositions and methods for silencing gene expression by delivering nucleic acid-lipid particles comprising a siRNA molecule to a cell.外源的 exogenous内源的 endo

14、genous靶 target基因组 genome基因失活 gene inactivation整合 integration外源核酸序列的靶向整合和表达 本发明公开了将外源序列靶向整合到基因组中预定靶位点，以(例如)用于蛋白质表达和基因失活的方法和组合物。Targeted integration and expression of exogenous nucleic acid sequences Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into a

15、 predetermined target site in a genome for use, for example, in protein expression and gene inactivation.同源的homologous异源的heterologous本发明涉及新的表达系统，其中用日本曲霉型种为宿主细胞表达异源蛋白质。The present invention relates to a novel expression system in which A. japonicus-type species are used as host cells for expression o

16、f heterologous proteins.由多个嵌合pRNA分子形成的多价多聚复合物，各携带至少一个生物学活性部分、可检测标记或其他异源组件。A polyvalent multimeric complex formed from a plurality of chimeric pRNA molecules, each carrying at least one biologically active moiety, detectable label, or other heterologous component.转录transcription/transcribe转录物/转录本 tra

17、nscript顺式 cis反式 trans本发明提供了能够产生预定大小的RNA转录物的重组转录单位，其包括与含有转录区域的核苷酸序列可操作连接的调节序列，从而，所述转录区域的转录受到所述调节序列的调控。所述转录区域包括编码病毒序列的区域和位于编码所述病毒序列的区域下游的非编码区域，其中所述非编码区域包括编码顺式作用核酶的核苷酸序列。也提供了应用这种重组转录单位的方法和包括含有这种重组转录单位的载体的细胞。The present invention provides a recombinant transcription unit capable of producing an RNA tran

18、script of a predetermined size comprising a regulatory sequence operably linked to a nucleotide sequence comprising a transcribed region such that the transcription of said transcribed region is controlled by said regulatory sequence. The transcribed region comprises a region that encodes for a vira

19、l sequence, and a non-coding region downstream of the region encoding for said viral sequence, wherein the non-coding region comprises a nucleotide sequence encoding a cis-acting ribozyme. Methods of using the recombinant transcription unit, and cells containing vectors comprising the recombinant tr

20、anscription unit are also disclosed.试剂盒 kit还公开了其用途，尤其从水性溶液分离核酸的方法，以及用于实施这些方法的试剂盒。The use thereof, particularly methods for the isolation of nucleic acids from an aqueous solution, as well as kits for performing those methods are disclosed.本发明涉及用于从样品中纯化RNA、DNA或两者的试剂、方法和试剂盒。 The invention features rea

21、gents, methods and kits for the purification of RNA, or DNA, or both, from a sample.衔接子 adaptorcDNA文库 cDNA library3偏倚 3 bias高通量 throughput提供了使用与自动化测序系统相容的衔接子序列，用于使mRNA样品高通量加工成cDNA文库的新生物化学规程。所提供的方法生产没有与目前cDNA文库生产方法相关的3偏倚的cDNA文库。New biochemical protocols for high throughput processing of mRNA samples

22、into cDNA libraries with adaptor sequences compatible with automated sequencing systems are provided. The provided methods produces cDNA libraries which do not have 3 bias associated with current cDNA library production methods. 模板 template本发明涉及利用含有作为多样性模板的序列区域的核酸分子使核酸序列多样化的方法。因此，本发明提供了待多样化的核酸分子，以及用

23、作模板区(TR)和与TR协同进行定向定点多样化的核酸分子。This invention relates to the diversification of nucleic acid sequences by use of a nucleic acid molecule containing a region of sequence that acts as a template for diversification. The invention thus provides nucleic acid molecules to be diversified, as well as those w

24、hich act as the template region (TR) and in concert with the TR for directional, site-specific diversification. 正义链 sense strand/positive stand反义链 antisense strand/negative strand过表达 overexpression提供了针对Apo-B100基因的寡核苷酸来调节Apo-B100的表达。组合物包含寡核苷酸，尤其是反义寡核苷酸，其靶向编码Apo-B100的核酸。提供了使用这些化合物调节Apo-B100表达和治疗与Apo-B

25、100过表达、突变Apo-B100过表达或二者相关的疾病的方法。Oligonucleotides directed against the Apo-B100 gene are provided for modulating the expression of Apo-B100. The compositions comprise oligonucleotides, particularly antisense oligonucleotides, targeted to nucleic acids encoding the Apo-Bl00. Methods of using these com

26、pounds for modulation of Apo-Bl00 expression and for the treatment of diseases associated with either overexpression of Apo-Bl00, expression of mutated Apo-Bl00 or both are provided. 101273142A用于癌症治疗的调节SMYD3转移酶的化合物的鉴定优先权信息:US20050695957P/pr本发明涉及利用测定多肽的甲基转移酶活性以及筛选甲基转移酶活性调节剂，更具体为SMYD3对视网膜母细胞瘤的甲基化的调节剂的

27、方法。本发明进一步提供了利用如此鉴定的调节剂预防或治疗癌症的方法或药物组合物，所述癌症包括结肠直肠癌、肝细胞癌、膀胱癌和/或乳腺癌。SMYD3 (又称ZNFN3A1)具有更高的甲基化活性。Lys824是RB1蛋白上优选的 SMYD3甲基化位点。The present invention features a method for determining the methyltransferas e activity of a polypeptide and screening for modulators of methyltransferase activity, more particul

28、arly for modulators of the methylation of retinoblastoma by SMYD3. The invention further provides a method or pharmaceutical composition for prevention or treating of colorectal cancer, hepatocellular carcinoma, bladder cancer and/or breast cancer using a modulator so identified. N-terminal truncate

29、d forms of SMYD3 (alias ZNFN3A1) have higher methylating activity. Lys 824 is a preferred methylation site on the RB1 protein for SMYD3.101273143A用于遗传序列分析的多重聚合酶链式反应优先权信息:US20050691768P/pr本发明涉及PCR方法，其包括：提供怀疑含有一种或多种病原体核酸的生物样品；添加多种与病原体内找得到的基因相对应的PCR引物；以及在样品上进行聚合酶链式反应以扩增与基因相对应的核酸的亚群。所述引物包括用于每种病原体的至少一种引物

30、对，并且引物含有与任何病原体DNA或与样品内任何背景DNA不同源的尾随序列。聚合酶链式反应内至少一种引物的浓度不大于约100nM。A PCR method involving: providing a biological sample suspected of containing one or more pathogen nucleic acids; adding a plurality of PCR primers corresponding to genes found in the pathogens; and performing a polymerase chain reacti

31、on on the sample to amplify a subset of the nucleic acids that correspond to the genes. The primers include at least one primer pair for each pathogen, and the primers contain a tail sequence that is not homologous any pathogen DNA or to any background DNA in the sample. The concentration of at leas

32、t one primer in the polymerase chain reaction is no more than about 100 nM.101273144A诊断食道癌的方法优先权信息:US20050703263P/pr为了鉴定涉及食道癌发生的分子和可用作诊断标志物及新药及免疫疗法的靶标的分子，构建了代表32,256个基因的cDNA微阵列，用来分析了通过激光捕获显微解剖纯化的19个食道鳞状细胞癌(ESSCCS)的表达模式。本文公开了在食道癌中显著上调或下调的基因群的详细全基因组数据。这些基因可用于开发治疗药物或免疫疗法以及肿瘤标志物。另外，本文公开了与淋巴结转移和手术后复发相关的基因。在候选的分子靶标基因中，进一步表征了人上皮细胞转化序列2癌基因(ECT2)和细胞分裂周期蛋白 45，酿酒酵母类同源物