Different Chinese herbal medicines the toxic components manshuriensis aristolochic acid A content毕.docx

Different Chinese herbal medicines the toxic components manshuriensis aristolochic acid A content毕.docx

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DifferentChineseherbalmedicinesthetoxiccomponentsmanshuriensisaristolochicacidAcontent毕

DifferentChineseherbalmedicinesthetoxiccomponentsmanshuriensisaristolochicacidAcontent

ComparemanshuriensisandLongDanXieGantrendofaristolochicacidAdemolitionpartygroupofChineseherbalmedicinesonthecontentofaristolochicacidA[method][Abstract]observedproportionofLongdanoriginalsideforthetestsamplepointssevengroups:

GuanMuTonggroup,fullpartygroupmanshuriensis+Qingregroup,manshuriensis+Ziyingroup,GuanMuTong+Leewatergroupmanshuriensis+licoricegroup,negativecontrolgroup（afullgooffwoodpass,RP-HPLCchromatography（RPHPLCmethodtoDiamonsilTM（diamonds）C18（250mm×4.6mm,of5μmcolumnwithmethanol-water-glacialaceticacid（volumeratio74:

24:

1）asthemobilephase,detectionwavelength315nmweremeasuredaristolochicacidAinthegroup.[Results]ComparedwithmanshuriensisgroupLongdanprescriptionlowestcontentofaristolochicacidAmanshuriensis+ZiyingrouparistolochicacidAcontentwassignificantlyreduced.[Conclusion]undertheguidanceofthetheoryofprescriptioncompatibilitythereasonablecompatibilityitisfeasibletoreducethetoxicityofmanshuriensis.

[Keywords]manshuriensischemicalLongdanaristolochicacidAanalysisofChineseherbalmedicineschromatographyhighperformanceliquidTherelatedwoodthepass（ofCaulisAristolochiaeManshuriensis,CAMtheAristolochicofAristolochiamanshruiensisplantnortheastofaristolochicKom.Driedcane,itsmainchemicalcomponentsofaristolochicacidA,B,D,thearistolochicDether,Magnoliaalkalioleanolicacid[1]thebittercoldtoxicmanshuriensis,pureheartandfire,Leeurine,passthroughtheeffectofmilkformouthsores,upsetredurine,edema,hotshowerastringentpain,vaginaldischarge,amenorrhealessmilk,hotandhumidTongbietc.Modernresearchshowsthatitspharmacologicaleffectsdiuretic,anti-mutagenic,anti-tumorandbloodpressureloweringeffect[2].

Manshuriensiscontainaristolochicaciddamagetohumankidneys,theStateFoodandDrugAdministrationissuedanoticeinJune2003tocancelitsmedicinalstandardThismeansmanshuriensis,willneverbeabletouseasalegitimateformoftraditionalChinesemedicine.AsuccessiveapplicationmostfamoussquareLongdan,（LongdanXieganDecoction,LXD,forexample,toobservewhetherChineseherbalmedicinescanreducemanshuriensistoxicitytoverifytheprescriptioncompositiontheoryofrationality.

Variorum>>From<<LongDanXieGanmedicalside,diarrheathehepatobiliaryrealfire,clearedunderthenameoffocushumidsquareontheinflammationtheattendinghepatobiliaryrealfire,liverandgallbladderdampbetcard.Inrecentyears,athomeandabroadcontinuetohavereportedtakingLongdanxieganleadtokidneydamage,andmoreirrationaluseoflong-termover-taking[3-5]LongDanXieGanapplicationsforhundredsofyearsbutithasnotadversereactionstotheexperimentbyreversed-phasehighperformanceliquidchromatography（RPHPLC）methodobservecontentLongdanitsdemolitionpartygrouponmanshuriensisaristolochicacidA（adritolochicacid,AA）,toexplorethetraditionalChinesemedicinecompatibilityreducemanshuriensistoxicityoflaw.

1instrumentandreagent1.1InstrumentDionexSummitP680highperformanceliquidchromatograph（AmericanDIONEX）theChromeleonchromatographyworkstation（USADIONEX,UVD170UUVdetector（theUnitedStatesDIONEXcompany.

1.2DrugsReferencesubstancearistolochicacidAisprovidedbytheNationalDrugandBiologicalProducts（batchnumber:

110746200406gentian,skullcap,gardenia,Alisma,angelica,rehmannia,Plantago,Bupleurum,rawlicoriceScrophulariaceae,OphiopogonpurchasedfrompharmaciesFirstAffiliatedHospitalofGuangzhouUniversityofTraditionalChineseMedicine,GuanMuTongispurchasedinHebeianguomedicinemarket,TCMEvaluationDepartmentoftheGuangzhouUniversityofTraditionalChineseMedicineProfessorLiWeiidentificationofAristolochiamanshruiensisKom..

1.3ReagentsMethanolHPLCgrade,glacialaceticacid,anhydrousmethanol,hydrochloricacidandetherwereofanalyticalgrade,waterpurewaterC’estbon.

2MethodsandResults2.1ChromatographicconditionsColumn:

DiamonsilTM（diamonds）C18（250mm×4.6mm,5μmdetector:

UVD170UUVdetection,detectionwavelength:

315nm,columntemperature:

roomtemperature（25°C）,mobilephase:

methanol-water-aceticacid（volumeratio75:

24:

1;theflowrate1mL/min,injectionvolume5μL.

2.2PreparationofsamplesUnderthemedicalsideVariorum>>Note:

“thegentianofdiarrheaJueyinofhot,theBupleurumflatShaoyangheat,skullcap,thegardeniaofQingfeiwithsanjiaoheattorankandfileofAlismadiarrheakidneythroughthewetwoodpass,thePlantagodiarrheabladderwetrankandfileofthedownwardspiralareasbittercolddrug,sobynormalization,andnourishingandBugan,thelicoricetoslowwithoutinjuryinthestomach,sotheminister“willFangzhongLonggallgrass,Bupleurum,Scutellaria,Gardeniaisclassifiedasantipyretic,willAlisma,PlantagonormalizedordertofacilitatewatermedicineAngelica,habitatclassifiedasYinanddrugdosesproportionoftheoriginalsquare,dividedintosevengroups:

manshuriensisgroup:

OffAkebia3g,all-partygroup:

OffAkebia3g,gentian2g,theskullcap3g,thegardenia3g,Alisma4g,theAngelica1g,Rehmanniae3gPlantago3gBupleurum2g,rawlicorice2g,manshuriensis+QingreGroup:

GuanMuTong3g,2ggentian,yellowbaicalensis3g,gardenia3g,Bupleurum2g,manshuriensis+Ziyingroup:

GuanMuTong3g,Angelica1g,Rehmanniae3g,manshuriensis+Leewatergroup:

GuanMuTong3g,Alisma4g,carformersub-3g,manshuriensis+licoricegroup:

OffAkebia3g,rawlicorice2g,negativecontrolgroup:

gentian2g,theskullcap3g,thegardenia3g,Alisma4g,Angelica1g,therehmannia3g,thePlantago3g,Bupleurum2g,therawlicorice2g.

Forthetestsample,addingwater400mL,soaking30min,boiling60min,andfilteredwithgauze.Dregspluswater100mlboiling30mingauzefilter.Merge2timesthefiltratewasconcentratedto50mL,thelessons10mL,with6mol/LhydrochloricpH=2,transferredtoaseparatoryfunnelandextractedwithetherfourtimesby[6]（10mL,5ml×3,extractswerecombined,waterbathandevaporatedtodryness,dissolvedinmethanol,andconstantvolumeina10mLvolumetricflask.through0.45μmofmicroporousmembranefiltration,thatis,too.

2.3PreparationofthereferencesolutionAccuratelyweighedaristolochicacidAreferencesubstance,placedina10mLvolumetricflask,dissolvedinmethanol,anddilutetothemark,shake,thepreciseamountof1mLhome10mLvolumetricflask,dissolvedinmethanol,anddilutetovolume,theshakemade0.034mg/mLsolutionthatwas.

2.4linearrelationshipbetweenInaccordancewiththeabovechromatographicconditions,accuratelyweighedaristolochicacidAreferencesolution2.5,5,10,15,20μLpeakareaabscissaXinjectionvolumeordinateYstandardcurveresultshorseAristolochicacidlinearrangeof0.084~0.680μg,equationY=0.0446X-0.0173,R=0.9998（Figure1.

2.5precisiontestTakearistolochicacidAstandardsolutionconsecutiveinjectionsof5times,measuredaristolochicacidApeakareasR1.8%.

2.6StabilityTestTheresultsshowedthataristolochicacidAgoodstabilitywithin6h0,2,4,6hinjection4timespasssamplesunderthesameconditions,themeasuredpeakarea.AristolochicacidAcontentsR2.6%.Fetchtherelevantwood.

2.7RepeatabilitytestSameof1partmanshuriensissamples,samplemeasurementmethodsamebelowoperation,parallelmeasuredthreetimestocalculatethecontentofaristolochicacidA1.7%sR.

2.8TheaveragerecoveryexperimentPrecisionWeightakesamplesofknowncontentofthesample,respectively,addingacertainamountofaristolochicacidA2.2operationwiththedetermination,andtheamountbasedonthecontentofthesampleandthereferencesubstanceofaristolochicacidAcalculationofrecovery.anaveragerecoveryof99.3%ofthesampledeterminationLinkstofreepapersDownloadCenter

2.9

Ofeachgroupthesamplesolution,2.1ChromatographicconditionsweremeasuredandtheresultsareshowninTable1,Figure2.

Table1show,Longdandifferentcompatibilities,manshuriensisaristolochicacidAcontentcomparisonareasfollows:

afullgroup<manshuriensis+Ziyingroup<OffAkebia+Leewatergroup<manshuriensis+Qingregroup<offwoodthroughgroup<manshuriensis+licoricegroupshowthattheall-partygroupofaristolochicacidAdecreasedup.Table1LongdananddemolitionpartygroupofaristolochicacidAcontent

3DiscussionLongdanclinicalusehasbeenhundredsofyearsofhistory.Ancientmedicalpracticeoflong-term,mastercompatibilityrulemanshuriensis.,UndertheguidanceoftheChinesemedicinetheory,instrictaccordancewiththeLongdanindications,dosageandprecautions,rationaluse,youcanreduceoravoidtheoccurrenceofsideeffects[7].manshuriensisrenaltoxicityfromthetraditionalChinesemedicinetheoryintermsofbelongingdiuresisShangyinLongdancompatibilityZiyindrugs（habitat,AngelicatoraiseBuganbloodtotopreventdruginfiltrationLeeShangyin.manshuriensisZiyindrugscombinedtheresultsofthisexperimentcansignificantlyreducethecontentofaristolochicacidA,inlinewiththetraditionalChinesemedicinetheoryofcompatibility,undertheguidanceofTCMtheoryprescriptioncompatibilityreasonable.reportedintheliterature[8],thetreatmentresultsmanshuriensispoisoningcases,evenservedChinesemedicineLiuWeiDiHuangPilldisabledialysisinstablecond