GOODs缓冲液.docx
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GOODs缓冲液
GOOD's缓冲液
BufferReferenceCenter
UsefulpHRangesofSelectedBiologicalBuffers（25°C,0.1M）
Buffers
UsefulpHRange
pKa
（at20）
pKa
（at25）
pKa
（at37）
ReagentGrade
BPC
Grade
Ultra
Grade
MES
5.5–6.7
6.16
6.10
5.97
M8250
M2933
M5287
Bis-Tris
5.8–7.2
n/a
6.50
6.36
B9754
B4429
B7535
ADA
6.0–7.2
6.65
6.59
6.46
A9883
n/a
A8074
aces
6.1–7.5
6.88
6.78
6.54
A9758
A3594
A7949
PIPES
6.1–7.5
6.80
6.76
6.66
P6757
P1851
P8203
MOPSO
6.2–7.6
n/a
6.90
6.75
M8389
n/a
n/a
Bis-TrisPropane
6.3–9.5
n/a
6.8,9.0
n/a
B6755
B4679
B9410
BES
6.4–7.8
7.17
7.09
6.90
B9879
B4554
B6420
MOPS
6.5–7.9
7.28
7.20
7.02
M1254
M3183
M5162
TES
6.8–8.2
7.50
7.40
7.16
T1375
T5691
T6541
HEPES
6.8–8.2
7.55
7.48
7.31
H3375
H4034
H7273
DIPSO
7.0–8.2
n/a
7.60
7.35
D9648
n/a
D0306
MOBS
6.9–8.3
n/a
7.60
n/a
M3295
n/a
n/a
TAPSO
7.0–8.2
n/a
7.60
7.39
T9269
T5566
T0432
Trizma
7.0–9.0
8.20
8.06
7.72
T1503
T6066
T6791
HEPPSO
7.1–8.5
n/a
7.80
6.66
H3137
n/a
n/a
POPSO
7.2–8.5
n/a
7.80
7.63
P3405
n/a
P7088
TEA
7.3–8.3
n/a
7.80
n/a
T1377
n/a
n/a
EPPS
7.3–8.7
n/a
8.00
n/a
E9502
E0276
E1894
Tricine
7.4–8.8
8.16
8.05
7.80
T0377
T5816
T9784
Gly-Gly
7.5–8.9
n/a
8.20
n/a
G1002
G3915
G7278
Bicine
7.6–9.0
8.35
8.26
8.04
B3876
n/a
B8660
HEPBS
7.6–9.0
n/a
8.30
n/a
H6903
n/a
n/a
TAPS
7.7-9.1
8.49
8.40
8.18
T5130
T5316
T9659
AMPD
7.8–9.7
n/a
8.80
n/a
A9754
n/a
A9074
TABS
8.2–9.6
n/a
8.90
n/a
T1302
n/a
n/a
AMPSO
8.3–9.7
n/a
9.00
9.10
A6659
n/a
A7585
CHES
8.6–10.0
9.55
9.49
9.36
C2885
n/a
C8210
CAPSO
8.9–10.3
n/a
9.60
9.43
C2278
n/a
C8085
AMP
9.0–10.5
n/a
9.70
n/a
A9879
n/a
A9199
CAPS
9.7–11.1
10.56
10.40
10.02
C2632
n/a
C6070
CABS
10.0–11.4
n/a
10.70
n/a
C5580
n/a
n/a
TrizmaBufferTable–pHvs.Temperature（三羟甲基氨基甲烷）
pHatTemperature
g/Lfor0.05MSolution
5°C
25°C
37°C
TrizmaHCL
TrizmaBase
7.76
7.20
6.91
7.02
0.67
7.89
7.30
7.02
6.85
0.80
7.97
7.40
7.12
6.61
0.97
8.07
7.50
7.22
6.35
1.18
8.18
7.60
7.30
6.06
1.39
8.26
7.70
7.40
5.72
1.66
8.37
7.80
7.52
5.32
1.97
8.48
7.90
7.62
4.88
2.30
8.58
8.00
7.71
4.44
2.65
8.68
8.10
7.80
4.02
2.97
8.78
8.20
7.91
3.54
3.34
8.88
8.30
8.01
3.07
3.70
8.98
8.40
8.10
2.64
4.03
9.09
8.50
8.22
2.21
4.36
9.18
8.60
8.31
1.83
4.65
9.28
8.70
8.42
1.50
4.90
9.36
8.80
8.51
1.23
5.13
9.47
8.90
8.62
0.96
5.32
9.56
9.00
8.70
0.76
5.47
PhosphateBufferTable–0.2Msolution（磷酸盐缓冲液）
Potassium
PhosphateMonobasicAnhydrousg/L
SodiumPhosphate Dibasic
Heptahydrateg/L
23°CpH
PotassiumPhosphateMonobasicAnhydrousg/L
SodiumPhosphate
DibasicHeptahydrateg/L
23°CpH
22.4
3.49
5.7
10.80
29.51
6.9
22.08
4.29
5.8
9.36
32.73
7.0
21.60
5.37
5.9
7.92
35.95
7.1
21.05
6.60
6.0
6.72
38.63
7.2
20.40
8.05
6.1
5.52
41.31
7.3
19.56
9.93
6.2
4.56
43.46
7.4
18.60
12.07
6.3
3.84
45.07
7.5
17.64
14.22
6.4
3.12
46.68
7.6
16.44
16.90
6.5
2.52
48.55
7.7
15.00
20.12
6.6
2.04
49.09
7.8
13.56
23.34
6.7
1.68
49.89
7.9
12.24
26.29
6.8
1.27
50.81
8.0
CitricAcid–Na2HPO4BufferSolutions,pHapprox.2.6–7.6
Citricacidmonohydrate,C6H8O7•H2O,M.wt.210.14;0.1M-solutioncontains21.01g/l.Na2HPO4,M.wt.141.98;0.2M-solutioncontains28.40g/l,orNa2HPO4 •2H2O,M.wt.178.05;0.2M-solutioncontains35.61g/l.
xml0.1M-citricacidandyml0.2M-Na2HPO4 mixed.
pH
xml0.1M-citricacid
yml0.2-Na2HPO4
2.6
89.10
10.90
2.8
84.15
15.85
3.0
79.45
20.55
3.2
75.30
24.70
3.4
71.50
28.50
3.6
67.80
32.20
3.8
64.50
35.50
4.0
61.45
38.55
4.2
58.60
41.40
4.4
55.90
44.10
4.6
53.25
46.75
4.8
50.70
49.30
5.0
48.50
51.50
5.2
46.40
53.60
5.4
44.25
55.75
5.6
42.00
58.00
5.8
39.55
60.45
6.0
36.85
63.15
6.2
33.90
66.10
6.4
30.75
69.25
6.6
27.25
72.75
6.8
22.75
77.25
7.0
17.65
82.35
7.2
13.05
86.95
7.4
9.15
90.85
7.6
6.35
93.65
CitricAcid–SodiumCitrateBufferSolutions,pH3.0–6.2
Citricacidmonohydrate,C6H8O7 •H2O,M.wt.210.14;0.1M-solutioncontains21.01g/l.Trisodiumcitratedihydrate,C6H5O7Na3 •2H2O,M.wt.294.12;0.1M-solutioncontains29.41g/l.
xml0.1M-citricacidandyml0.1M-trisodiumcitratemixed.
pH
xml0.1M-citricacid
yml0.1M-trisodiumcitrate
3.0
82.0
18.0
3.2
77.5
22.5
3.4
73.0
27.0
3.6
68.5
31.5
3.8
63.5
36.5
4.0
59.0
41.0
4.2
54.0
46.0
4.4
49.5
50.5
4.6
44.5
55.5
4.8
40.0
60.0
5.0
35.0
65.0
5.2
30.5
69.5
5.4
25.5
74.5
5.6
21.0
79.0
5.8
16.0
84.0
6.0
11.5
88.5
6.2
8.0
92.0
SodiumAcetate–AceticAcidBufferSolutions,pH3.7–5.6
Sodiumacetatetrihydrate,CH3COONa•3H2O,M.wt.136.09;0.2M-solutioncontains27.22g/l.
xml0.2M-NaOAcandyml0.2M-HOAcmixed.
pH,18°C
xml0.2M-NaOAc
yml0.2M-HOAc
3.7
10.0
90.0
3.8
12.0
88.0
4.0
18.0
82.0
4.2
26.5
73.5
4.4
37.0
63.0
4.6
49.0
51.0
4.8
59.0
41.0
5.0
70.0
30.0
5.2
79.0
21.0
5.4
86.0
14.0
5.6
91.0
9.0
Na2HPO4–NaH2PO4BufferSolutions,pH5.8–8.0at25°C
Na2HPO4 •2H2O,M.wt.178.05;0.2M-solutioncontains35.61g/l.Na2HPO4 •12H2O,M.wt.358.22;0.2M-solutioncontains71.64g/l.NaH2PO4 •H2O,M.wt.138.01;0.2M-solutioncontains27.6g/l.NaH2PO4 •2H2O,M.wt.156.03;0.2M-solutioncontains31.21g/l.
xml0.2M-Na2HPO4,yml0.2M-NaH2PO4;dilutedto100mlwithH2O.
pH,25°C
xml0.2M-Na2HPO4
yml0.2M-NaH2PO4
5.8
4.0
46.0
6.0
6.15
43.85
6.2
9.25
40.75
6.4
13.25
36.75
6.6
18.75
31.25
6.8
24.5
25.5
7.0
30.5
19.5
7.2
36.0
14.0
7.4
40.5
9.5
7.6
43.5
6.5
7.8
45.75
4.25
8.0
47.35
2.65
Imidazole（glyoxaline）–HClbuffersolutions,pH6.2–7.8at25°C1
Imidazole,C3H4N2,M.wt.68.08.
25ml0.2M-imidazole（13.62g/l）,xml0.2M-HCl,dilutedto100mlwithH2O.
pH,25°C
xml0.2M-HCl
6.2
21.45
6.4
19.9
6.6
17.75
6.8
15.2
7.0
12.15
7.2
9.3
7.4
6.8
7.6
4.65
7.8
3.0
SodiumCarbonate–SodiumBicarbonateBufferSolutions,pH9.2–10.81
Na2CO3•10H2O,M.wt.286.2;0.1M-solutioncontains28.62g/l.NaHCO3,M.wt.84.0;0.1M-solutioncontains8.40g/l.
xml0.1M-Na2CO3 andyml0.1M-Na2HCO3 mixed.
pH
xml0.1M-Na2CO3
yml0.1M-NaHCO3
20°C
37°C
9.2
8.8
10
90
9.4
9.1
20
80
9.5
9.4
30
70
9.8
9.5
40
60
9.9
9.7
50
50
10.1
9.9
60
40
10.3
10.1
70
30
10.5
10.3
80
20
10.8
10.6
90
10
HelpfulFormulas
Percentagebyweight（w/v）
（%bufferdesired/100） x finalbuffervolume（ml）=gofstartingmaterialneeded.
MolarSolutions
desiredmolarity x formulaweight x solutionfinalvolume（L）=gramsneeded
Henderson-HasselbachEquation
Reference
1.Dawson,R.M.C.;Elliot,D.C.;Elliot,W.H.;Jones,K.M. DataforBiochemicalResearch; 3rded.,OxfordSciencePubl.,1986.
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